Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 26514..27049 Replicon plasmid pCFSAN027350
Accession NZ_CP037942
Organism Escherichia coli strain CFSAN027350

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag EKI55_RS27880 Protein ID WP_001270417.1
Coordinates 26762..27049 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag EKI55_RS27875 Protein ID WP_001132895.1
Coordinates 26514..26765 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI55_RS27850 22364..22729 - 366 WP_032345630.1 hypothetical protein -
EKI55_RS27855 22811..23413 + 603 Protein_20 DDE-type integrase/transposase/recombinase -
EKI55_RS27860 23634..24596 - 963 WP_032345409.1 ParB/RepB/Spo0J family partition protein -
EKI55_RS27865 24593..25798 - 1206 WP_000817635.1 AAA family ATPase -
EKI55_RS27875 26514..26765 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EKI55_RS27880 26762..27049 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EKI55_RS27885 27048..27296 + 249 Protein_25 integrase core domain-containing protein -
EKI55_RS27895 27976..28694 + 719 Protein_26 RepB family plasmid replication initiator protein -
EKI55_RS27900 28778..29428 + 651 WP_000993931.1 IS66 family insertion sequence hypothetical protein -
EKI55_RS27905 29428..29775 + 348 WP_000624618.1 IS66 family insertion sequence element accessory protein TnpB -
EKI55_RS27910 29795..31366 + 1572 WP_000381416.1 IS66 family transposase -
EKI55_RS27915 31395..31664 + 270 Protein_30 protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / toxB / hlyD / hlyB / hlyA / hlyC 1..157534 157534
- inside IScluster/Tn - - 28778..40903 12125


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T120455 WP_001270417.1 NZ_CP037942:26762-27049 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T120455 NZ_CP037942:26762-27049 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT120455 WP_001132895.1 NZ_CP037942:26514-26765 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT120455 NZ_CP037942:26514-26765 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References