Detailed information of TA system TA120453

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5190391..5191228
• Replicon: chromosome
• Accession: NZ_CP037941
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: EKI55_RS26430
• Protein ID: WP_000854687.1
• Coordinates: 5190391..5190771 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: EKI55_RS26435
• Protein ID: WP_001443392.1
• Coordinates: 5190860..5191228 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS26400	5186033..5187874	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
EKI55_RS26405	5187953..5188459	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
EKI55_RS26415	5188759..5189604	-	846	WP_001274557.1	DUF4942 domain-containing protein	-
EKI55_RS26420	5189689..5189886	-	198	WP_000772026.1	DUF957 domain-containing protein	-
EKI55_RS26425	5189906..5190394	-	489	WP_001054228.1	hypothetical protein	-
EKI55_RS26430	5190391..5190771	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
EKI55_RS26435	5190860..5191228	-	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EKI55_RS26440	5191304..5191525	-	222	WP_000692312.1	DUF987 domain-containing protein	-
EKI55_RS26445	5191588..5192064	-	477	WP_001186728.1	RadC family protein	-
EKI55_RS26450	5192080..5192553	-	474	WP_001444656.1	antirestriction protein	-
EKI55_RS26455	5192647..5192892	-	246	WP_000680587.1	hypothetical protein	-
EKI55_RS26460	5192892..5193332	-	441	Protein_5057	DUF932 domain-containing protein	-
EKI55_RS26470	5193711..5194867	+	1157	WP_100699673.1	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	5140277..5199918	59641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T120453 WP_000854687.1 NZ_CP037941:c5190771-5190391 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T120453 NZ_CP037941:c5190771-5190391 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT120453 WP_001443392.1 NZ_CP037941:c5191228-5190860 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT120453 NZ_CP037941:c5191228-5190860 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTAGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3