Detailed information of TA system TA120449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4548288..4548913
• Replicon: chromosome
• Accession: NZ_CP037941
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EKI55_RS23250
• Protein ID: WP_000911330.1
• Coordinates: 4548288..4548686 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EKI55_RS23255
• Protein ID: WP_000450524.1
• Coordinates: 4548686..4548913 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS23230	4544166..4544366	+	201	WP_000383836.1	YpfN family protein	-
EKI55_RS23235	4544476..4545174	-	699	WP_000679812.1	esterase	-
EKI55_RS23240	4545248..4547263	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
EKI55_RS23245	4547278..4548141	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
EKI55_RS23250	4548288..4548686	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EKI55_RS23255	4548686..4548913	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EKI55_RS23260	4549067..4549780	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EKI55_RS23265	4549993..4551027	-	1035	WP_032345214.1	outer membrane protein assembly factor BamC	-
EKI55_RS23270	4551044..4551922	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EKI55_RS23275	4552068..4552640	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EKI55_RS23280	4552640..4553110	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T120449 WP_000911330.1 NZ_CP037941:c4548686-4548288 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T120449 NZ_CP037941:c4548686-4548288 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT120449 WP_000450524.1 NZ_CP037941:c4548913-4548686 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT120449 NZ_CP037941:c4548913-4548686 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33