Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3699304..3699736 Replicon chromosome
Accession NZ_CP037941
Organism Escherichia coli strain CFSAN027350

Toxin (Protein)


Gene name parE_1 Uniprot ID -
Locus tag EKI55_RS18880 Protein ID WP_050439653.1
Coordinates 3699497..3699736 (+) Length 80 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EKI55_RS18875 Protein ID WP_001302048.1
Coordinates 3699304..3699495 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI55_RS18825 3694382..3694600 - 219 WP_000683609.1 DUF4014 family protein -
EKI55_RS18830 3694728..3695039 - 312 WP_001289673.1 hypothetical protein -
EKI55_RS18835 3695032..3695259 - 228 WP_000699809.1 hypothetical protein -
EKI55_RS18840 3695256..3695537 - 282 WP_000603384.1 DNA-binding protein -
EKI55_RS18845 3695570..3696331 - 762 WP_000450872.1 DUF1627 domain-containing protein -
EKI55_RS18850 3696357..3697098 - 742 Protein_3607 ATP-binding protein -
EKI55_RS18855 3697105..3698067 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
EKI55_RS18860 3698090..3698515 - 426 WP_000693943.1 Rha family transcriptional regulator -
EKI55_RS18865 3698512..3698814 - 303 WP_000172738.1 transcriptional regulator -
EKI55_RS18870 3698912..3699283 + 372 WP_001169687.1 hypothetical protein -
EKI55_RS18875 3699304..3699495 + 192 WP_001302048.1 hypothetical protein Antitoxin
EKI55_RS18880 3699497..3699736 + 240 WP_050439653.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EKI55_RS18885 3700062..3700214 + 153 WP_001427316.1 DUF1391 family protein -
EKI55_RS18890 3700635..3700856 + 222 WP_000560218.1 killing protein KilR -
EKI55_RS18895 3700850..3701026 + 177 WP_000245534.1 hypothetical protein -
EKI55_RS18900 3701100..3701375 + 276 WP_001307773.1 hypothetical protein -
EKI55_RS18905 3701474..3704125 + 2652 WP_032346644.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' / espFu/tccP 3641187..3748118 106931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-75)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 80 a.a.        Molecular weight: 9114.45 Da        Isoelectric Point: 4.6470

>T120437 WP_050439653.1 NZ_CP037941:3699497-3699736 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSV

Download         Length: 240 bp

>T120437 NZ_CP037941:3699497-3699736 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT120437 WP_001302048.1 NZ_CP037941:3699304-3699495 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT120437 NZ_CP037941:3699304-3699495 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References