Detailed information of TA system TA120428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2754641..2755119
• Replicon: chromosome
• Accession: NZ_CP037941
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: EKI55_RS13715
• Protein ID: WP_001303876.1
• Coordinates: 2754641..2754928 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: EKI55_RS13720
• Protein ID: WP_000536233.1
• Coordinates: 2754928..2755119 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS13680	2749803..2752274	-	2472	WP_000048520.1	exonuclease	-
EKI55_RS13685	2752367..2752558	-	192	WP_001090200.1	DUF1482 family protein	-
EKI55_RS13690	2752555..2752743	-	189	WP_000449192.1	cell division inhibition protein DicB	-
EKI55_RS13700	2753306..2753491	+	186	WP_001133046.1	hypothetical protein	-
EKI55_RS13705	2753678..2754067	-	390	WP_000394511.1	hypothetical protein	-
EKI55_RS13710	2754209..2754364	-	156	WP_000379575.1	DUF1391 family protein	-
EKI55_RS13715	2754641..2754928	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EKI55_RS13720	2754928..2755119	-	192	WP_000536233.1	hypothetical protein	Antitoxin
EKI55_RS13725	2755147..2755548	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
EKI55_RS13730	2755657..2755929	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
EKI55_RS13735	2755913..2756338	+	426	WP_000693855.1	Rha family transcriptional regulator	-
EKI55_RS13740	2756544..2756999	-	456	WP_000273724.1	hypothetical protein	-
EKI55_RS13745	2757078..2758169	+	1092	WP_001205823.1	hypothetical protein	-
EKI55_RS13750	2758176..2758922	+	747	WP_000788750.1	ATP-binding protein	-
EKI55_RS13755	2758944..2759714	+	771	WP_000451007.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espW / espM2	2746557..2828184	81627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T120428 WP_001303876.1 NZ_CP037941:c2754928-2754641 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T120428 NZ_CP037941:c2754928-2754641 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT120428 WP_000536233.1 NZ_CP037941:c2755119-2754928 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT120428 NZ_CP037941:c2755119-2754928 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA