Detailed information of TA system TA120422

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1252847..1253679
• Replicon: chromosome
• Accession: NZ_CP037941
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: EKI55_RS06135
• Protein ID: WP_000854753.1
• Coordinates: 1253305..1253679 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: EKI55_RS06130
• Protein ID: WP_001315620.1
• Coordinates: 1252847..1253215 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS06095	1249683..1250138	+	456	WP_000581504.1	hypothetical protein	-
EKI55_RS06100	1250217..1250450	+	234	WP_001119729.1	DUF905 family protein	-
EKI55_RS06110	1250550..1251368	+	819	WP_001234622.1	DUF945 domain-containing protein	-
EKI55_RS06115	1251423..1251908	+	486	WP_000849588.1	antirestriction protein	-
EKI55_RS06120	1251924..1252400	+	477	WP_001186774.1	RadC family protein	-
EKI55_RS06125	1252463..1252684	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EKI55_RS06130	1252847..1253215	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EKI55_RS06135	1253305..1253679	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
EKI55_RS06140	1253676..1254164	+	489	WP_000777547.1	hypothetical protein	-
EKI55_RS06145	1254176..1254373	+	198	WP_000839281.1	DUF957 domain-containing protein	-
EKI55_RS28795	1254458..1254610	+	153	WP_001280445.1	hypothetical protein	-
EKI55_RS06170	1255655..1256305	+	651	WP_001037966.1	HNH endonuclease	-
EKI55_RS06175	1256313..1257293	-	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EKI55_RS06180	1257358..1258464	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T120422 WP_000854753.1 NZ_CP037941:1253305-1253679 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T120422 NZ_CP037941:1253305-1253679 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT120422 WP_001315620.1 NZ_CP037941:1252847-1253215 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT120422 NZ_CP037941:1252847-1253215 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5