Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4111326..4111945 Replicon chromosome
Accession NZ_CP037928
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP-8788

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag E2E32_RS20145 Protein ID WP_002892050.1
Coordinates 4111727..4111945 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag E2E32_RS20140 Protein ID WP_002892066.1
Coordinates 4111326..4111700 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2E32_RS20130 4106478..4107671 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E2E32_RS20135 4107694..4110840 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
E2E32_RS20140 4111326..4111700 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
E2E32_RS20145 4111727..4111945 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
E2E32_RS20150 4112104..4112670 + 567 WP_002892030.1 maltose O-acetyltransferase -
E2E32_RS28780 4112642..4112782 - 141 WP_004147370.1 hypothetical protein -
E2E32_RS20155 4112803..4113273 + 471 WP_002892026.1 YlaC family protein -
E2E32_RS20160 4113248..4114699 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
E2E32_RS20165 4114800..4115498 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
E2E32_RS20170 4115495..4115635 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
E2E32_RS20175 4115635..4115898 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T120411 WP_002892050.1 NZ_CP037928:4111727-4111945 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T120411 NZ_CP037928:4111727-4111945 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT120411 WP_002892066.1 NZ_CP037928:4111326-4111700 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT120411 NZ_CP037928:4111326-4111700 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References