Detailed information of TA system TA120396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4819535..4820143
• Replicon: chromosome
• Accession: NZ_CP037926
• Organism: Pseudomonas aeruginosa strain AES1R

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: CKAES1R_RS23265
• Protein ID: WP_019682344.1
• Coordinates: 4819535..4819882 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: CKAES1R_RS23270
• Protein ID: WP_003114155.1
• Coordinates: 4819892..4820143 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKAES1R_RS23235	4814954..4815226	+	273	WP_003120794.1	cysteine-rich CWC family protein	-
CKAES1R_RS23240	4815226..4815918	+	693	WP_003114159.1	16S rRNA pseudouridine(516) synthase	-
CKAES1R_RS23245	4816054..4817097	+	1044	WP_019682613.1	L,D-transpeptidase	-
CKAES1R_RS23250	4817177..4817914	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
CKAES1R_RS23255	4818306..4819208	+	903	WP_003085447.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
CKAES1R_RS23265	4819535..4819882	-	348	WP_019682344.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKAES1R_RS23270	4819892..4820143	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CKAES1R_RS23275	4820357..4821340	-	984	WP_019682343.1	tyrosine-type recombinase/integrase	-
CKAES1R_RS23280	4821340..4822632	-	1293	WP_003123045.1	hypothetical protein	-
CKAES1R_RS23285	4822891..4824153	-	1263	WP_054377817.1	hypothetical protein	-
CKAES1R_RS23290	4824155..4824505	-	351	WP_019682242.1	DUF2523 domain-containing protein	-
CKAES1R_RS30730	4824515..4825121	-	607	Protein_4481	coat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4819535..4841749	22214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12872.65 Da
• Isoelectric Point: 4.5143

>T120396 WP_019682344.1 NZ_CP037926:c4819882-4819535 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGAQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGAIE

Nucleotide

• Length: 348 bp

>T120396 NZ_CP037926:c4819882-4819535 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGCACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGGCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT120396 WP_003114155.1 NZ_CP037926:c4820143-4819892 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT120396 NZ_CP037926:c4820143-4819892 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355