Detailed information of TA system TA120386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1257393..1258001
• Replicon: chromosome
• Accession: NZ_CP037925
• Organism: Pseudomonas aeruginosa strain AES1M

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: CKAES1M_RS05965
• Protein ID: WP_019682344.1
• Coordinates: 1257654..1258001 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: CKAES1M_RS05960
• Protein ID: WP_003114155.1
• Coordinates: 1257393..1257644 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKAES1M_RS30570	1252415..1253021	+	607	Protein_1138	coat protein	-
CKAES1M_RS05940	1253031..1253381	+	351	WP_019682242.1	DUF2523 domain-containing protein	-
CKAES1M_RS05945	1253383..1254645	+	1263	WP_054377817.1	hypothetical protein	-
CKAES1M_RS05950	1254904..1256196	+	1293	WP_003123045.1	hypothetical protein	-
CKAES1M_RS05955	1256196..1257179	+	984	WP_019682343.1	tyrosine-type recombinase/integrase	-
CKAES1M_RS05960	1257393..1257644	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CKAES1M_RS05965	1257654..1258001	+	348	WP_019682344.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKAES1M_RS05975	1258328..1259230	-	903	WP_003085447.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
CKAES1M_RS05980	1259622..1260359	-	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
CKAES1M_RS05985	1260439..1261482	-	1044	WP_019682613.1	L,D-transpeptidase	-
CKAES1M_RS05990	1261618..1262310	-	693	WP_003114159.1	16S rRNA pseudouridine(516) synthase	-
CKAES1M_RS05995	1262310..1262582	-	273	WP_003120794.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	1235787..1267045	31258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12872.65 Da
• Isoelectric Point: 4.5143

>T120386 WP_019682344.1 NZ_CP037925:1257654-1258001 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGAQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGAIE

Nucleotide

• Length: 348 bp

>T120386 NZ_CP037925:1257654-1258001 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGCACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGGCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT120386 WP_003114155.1 NZ_CP037925:1257393-1257644 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT120386 NZ_CP037925:1257393-1257644 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355