Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 44096..44871 Replicon plasmid pWR100
Accession NZ_CP037924
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name tacT Uniprot ID Q7BEG9
Locus tag EKN05_RS23725 Protein ID WP_000405245.1
Coordinates 44389..44871 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag EKN05_RS23720 Protein ID WP_010921625.1
Coordinates 44096..44398 (+) Length 101 a.a.

Genomic Context

EKN05_RS23690EKN05_RS23720EKN05_RS23725EKN05_RS23730EKN05_RS23685EKN05_RS23700EKN05_RS23705EKN05_RS23710EKN05_RS23735EKN05_RS23740
Location: 40460..41914 (1455 bp)
Type: Others
Protein ID: WP_010921627.1
Location: 44096..44398 (303 bp)
Type: Antitoxin
Protein ID: WP_010921625.1
Location: 44389..44871 (483 bp)
Type: Toxin
Protein ID: WP_000405245.1
Location: 44939..45462 (524 bp)
Type: Others
Protein ID: Protein_50
Location: 39437..40056 (620 bp)
Type: Others
Protein ID: Protein_43
Location: 42063..42191 (129 bp)
Type: Others
Protein ID: Protein_45
Location: 42363..43571 (1209 bp)
Type: Others
Protein ID: WP_010921626.1
Location: 43552..43824 (273 bp)
Type: Others
Protein ID: WP_000019158.1
Location: 45508..46609 (1102 bp)
Type: Others
Protein ID: Protein_51
Location: 46661..48352 (1692 bp)
Type: Others
Protein ID: WP_010921624.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
EKN05_RS23685 (EKN05_023705)39437..40056-620Protein_43IS1 family transposase-
EKN05_RS23690 (EKN05_023710)40460..41914+1455WP_010921627.1type 3 secretion system effector OspC2-
EKN05_RS2370042063..42191-129Protein_45IS5/IS1182 family transposase-
EKN05_RS23705 (EKN05_023725)42363..43571-1209WP_010921626.1IS91 family transposase-
EKN05_RS23710 (EKN05_023730)43552..43824-273WP_000019158.1hypothetical protein-
EKN05_RS23720 (EKN05_023740)44096..44398+303WP_010921625.1DUF1778 domain-containing proteinAntitoxin
EKN05_RS23725 (EKN05_023745)44389..44871+483WP_000405245.1GNAT family N-acetyltransferaseToxin
EKN05_RS23730 (EKN05_023750)44939..45462+524Protein_50IS3-like element ISSfl11 family transposase-
EKN05_RS23735 (EKN05_023755)45508..46609-1102Protein_51transposase-
EKN05_RS23740 (EKN05_023760)46661..48352-1692WP_010921624.1T3SS effector E3 ubiquitin-protein ligase IpaH2.5-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideNon-Mobilizable plasmid-ospC2 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA1..232195232195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17750.52 Da        Isoelectric Point: 7.0382

>T120380 WP_000405245.1 NZ_CP037924:44389-44871 [Shigella flexneri 5a str. M90T]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T120380 NZ_CP037924:44389-44871 [Shigella flexneri 5a str. M90T]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT120380 WP_010921625.1 NZ_CP037924:44096-44398 [Shigella flexneri 5a str. M90T]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT120380 NZ_CP037924:44096-44398 [Shigella flexneri 5a str. M90T]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBQ7BEG9

Antitoxin

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Source
ID
Structure
AlphaFold DBQ7BEG8

References