Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 5267..6024 Replicon plasmid pWR100
Accession NZ_CP037924
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name tacT Uniprot ID Q99QI3
Locus tag EKN05_RS23485 Protein ID WP_010921647.1
Coordinates 5539..6024 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag EKN05_RS23480 Protein ID WP_011114751.1
Coordinates 5267..5551 (+) Length 95 a.a.

Genomic Context

EKN05_RS23445EKN05_RS23450EKN05_RS23455EKN05_RS23480EKN05_RS23485EKN05_RS23495EKN05_RS23500EKN05_RS23505EKN05_RS23510EKN05_RS23515EKN05_RS23520EKN05_RS25285EKN05_RS23490
Location: 1333..2007 (675 bp)
Type: Others
Protein ID: WP_010921654.1
Location: 2004..2354 (351 bp)
Type: Others
Protein ID: WP_005048975.1
Location: 2351..3947 (1597 bp)
Type: Others
Protein ID: Protein_3
Location: 5267..5551 (285 bp)
Type: Antitoxin
Protein ID: WP_011114751.1
Location: 5539..6024 (486 bp)
Type: Toxin
Protein ID: WP_010921647.1
Location: 6545..7701 (1157 bp)
Type: Others
Protein ID: WP_136350434.1
Location: 7816..9044 (1229 bp)
Type: Others
Protein ID: WP_094081542.1
Location: 9082..9225 (144 bp)
Type: Others
Protein ID: Protein_10
Location: 9210..9848 (639 bp)
Type: Others
Protein ID: WP_000502855.1
Location: 10076..10462 (387 bp)
Type: Others
Protein ID: Protein_12
Location: 10493..10966 (474 bp)
Type: Others
Protein ID: WP_005038443.1
Location: 4201..4365 (165 bp)
Type: Others
Protein ID: WP_001346193.1
Location: 6197..6478 (282 bp)
Type: Others
Protein ID: Protein_7
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
EKN05_RS23445 (EKN05_023455)1333..2007+675WP_010921654.1IS66-like element accessory protein TnpA-
EKN05_RS23450 (EKN05_023460)2004..2354+351WP_005048975.1IS66 family insertion sequence element accessory protein TnpB-
EKN05_RS23455 (EKN05_023465)2351..3947+1597Protein_3IS66-like element ISSfl3 family transposase-
EKN05_RS252854201..4365-165WP_001346193.1hypothetical protein-
EKN05_RS23480 (EKN05_023490)5267..5551+285WP_011114751.1DUF1778 domain-containing proteinAntitoxin
EKN05_RS23485 (EKN05_023495)5539..6024+486WP_010921647.1GNAT family N-acetyltransferaseToxin
EKN05_RS23490 (EKN05_023500)6197..6478-282Protein_7IS4/IS5 family transposase-
EKN05_RS23495 (EKN05_023505)6545..7701+1157WP_136350434.1IS3-like element IS600 family transposase-
EKN05_RS23500 (EKN05_023510)7816..9044+1229WP_094081542.1IS3-like element IS2 family transposase-
EKN05_RS23505 (EKN05_023515)9082..9225+144Protein_10DUF3440 domain-containing protein-
EKN05_RS23510 (EKN05_023520)9210..9848+639WP_000502855.1ParB N-terminal domain-containing protein-
EKN05_RS23515 (EKN05_023525)10076..10462+387Protein_12hypothetical protein-
EKN05_RS23520 (EKN05_023530)10493..10966+474WP_005038443.1AraC family transcriptional regulator-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideNon-Mobilizable plasmid-ospC2 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA1..232195232195
-insideIScluster/Tn-ospC1 / ospD3/senA / ipaH4.5 / ipaH7.81333..3061629283


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17784.60 Da        Isoelectric Point: 9.8822

>T120379 WP_010921647.1 NZ_CP037924:5539-6024 [Shigella flexneri 5a str. M90T]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T120379 NZ_CP037924:5539-6024 [Shigella flexneri 5a str. M90T]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT120379 WP_011114751.1 NZ_CP037924:5267-5551 [Shigella flexneri 5a str. M90T]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT120379 NZ_CP037924:5267-5551 [Shigella flexneri 5a str. M90T]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBA0A4P7TUC3

Antitoxin

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Source
ID
Structure
AlphaFold DBA0A4V1CUJ1

References