Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 5267..6024 Replicon plasmid pWR100
Accession NZ_CP037924
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name tacT Uniprot ID Q99QI3
Locus tag EKN05_RS23485 Protein ID WP_010921647.1
Coordinates 5539..6024 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag EKN05_RS23480 Protein ID WP_011114751.1
Coordinates 5267..5551 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN05_RS23445 (EKN05_023455) 1333..2007 + 675 WP_010921654.1 IS66-like element accessory protein TnpA -
EKN05_RS23450 (EKN05_023460) 2004..2354 + 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
EKN05_RS23455 (EKN05_023465) 2351..3947 + 1597 Protein_3 IS66-like element ISSfl3 family transposase -
EKN05_RS25285 4201..4365 - 165 WP_001346193.1 hypothetical protein -
EKN05_RS23480 (EKN05_023490) 5267..5551 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
EKN05_RS23485 (EKN05_023495) 5539..6024 + 486 WP_010921647.1 GNAT family N-acetyltransferase Toxin
EKN05_RS23490 (EKN05_023500) 6197..6478 - 282 Protein_7 IS4/IS5 family transposase -
EKN05_RS23495 (EKN05_023505) 6545..7701 + 1157 WP_136350434.1 IS3-like element IS600 family transposase -
EKN05_RS23500 (EKN05_023510) 7816..9044 + 1229 WP_094081542.1 IS3-like element IS2 family transposase -
EKN05_RS23505 (EKN05_023515) 9082..9225 + 144 Protein_10 DUF3440 domain-containing protein -
EKN05_RS23510 (EKN05_023520) 9210..9848 + 639 WP_000502855.1 ParB N-terminal domain-containing protein -
EKN05_RS23515 (EKN05_023525) 10076..10462 + 387 Protein_12 hypothetical protein -
EKN05_RS23520 (EKN05_023530) 10493..10966 + 474 WP_005038443.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA 1..232195 232195
- inside IScluster/Tn - ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 1333..30616 29283


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17784.60 Da        Isoelectric Point: 9.8822

>T120379 WP_010921647.1 NZ_CP037924:5539-6024 [Shigella flexneri 5a str. M90T]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T120379 NZ_CP037924:5539-6024 [Shigella flexneri 5a str. M90T]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT120379 WP_011114751.1 NZ_CP037924:5267-5551 [Shigella flexneri 5a str. M90T]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT120379 NZ_CP037924:5267-5551 [Shigella flexneri 5a str. M90T]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TUC3

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References