Detailed information of TA system TA120376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2802164..2803008
• Replicon: chromosome
• Accession: NZ_CP037923
• Organism: Shigella flexneri 5a str. M90T

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: EKN05_RS14565
• Protein ID: WP_014532172.1
• Coordinates: 2802547..2803008 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A4P7TQW7
• Locus tag: EKN05_RS14555
• Protein ID: WP_024260230.1
• Coordinates: 2802164..2802475 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKN05_RS14530 (EKN05_014545)	2797634..2798131	+	498	Protein_2795	COX aromatic rich motif-containing protein	-
EKN05_RS14535 (EKN05_014550)	2798153..2800144	+	1992	Protein_2796	cytochrome o ubiquinol oxidase subunit I	-
EKN05_RS14540 (EKN05_014555)	2800134..2800748	+	615	Protein_2797	cytochrome o ubiquinol oxidase subunit III	-
EKN05_RS14545 (EKN05_014560)	2800748..2801077	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EKN05_RS14550 (EKN05_014565)	2801089..2801979	+	891	Protein_2799	heme o synthase	-
EKN05_RS14555 (EKN05_014570)	2802164..2802475	+	312	WP_024260230.1	DUF1778 domain-containing protein	Antitoxin
EKN05_RS14565 (EKN05_014580)	2802547..2803008	+	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
EKN05_RS14570 (EKN05_014585)	2803064..2803999	-	936	WP_005105029.1	tetratricopeptide repeat protein	-
EKN05_RS14575 (EKN05_014590)	2804010..2804243	-	234	WP_128600802.1	hypothetical protein	-
EKN05_RS14580 (EKN05_014595)	2804236..2805510	-	1275	WP_005105027.1	tetratricopeptide repeat protein	-
EKN05_RS14585 (EKN05_014600)	2805904..2807274	+	1371	WP_001000961.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T120376 WP_014532172.1 NZ_CP037923:2802547-2803008 [Shigella flexneri 5a str. M90T]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T120376 NZ_CP037923:2802547-2803008 [Shigella flexneri 5a str. M90T]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11440.14 Da
• Isoelectric Point: 10.3388

>AT120376 WP_024260230.1 NZ_CP037923:2802164-2802475 [Shigella flexneri 5a str. M90T]
MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIT
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT120376 NZ_CP037923:2802164-2802475 [Shigella flexneri 5a str. M90T]
ATGCTTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCACT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TQW7