Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2764543..2765161 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EKN05_RS14370 Protein ID WP_001291435.1
Coordinates 2764943..2765161 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag EKN05_RS14365 Protein ID WP_000344797.1
Coordinates 2764543..2764917 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN05_RS14355 (EKN05_014365) 2759632..2760825 + 1194 WP_024260231.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EKN05_RS14360 (EKN05_014370) 2760848..2763997 + 3150 WP_001132471.1 efflux RND transporter permease AcrB -
EKN05_RS14365 (EKN05_014375) 2764543..2764917 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
EKN05_RS14370 (EKN05_014380) 2764943..2765161 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EKN05_RS14375 (EKN05_014385) 2765333..2765884 + 552 WP_000102569.1 maltose O-acetyltransferase -
EKN05_RS14380 (EKN05_014390) 2766000..2766470 + 471 WP_000136192.1 YlaC family protein -
EKN05_RS14385 (EKN05_014395) 2766634..2768184 + 1551 WP_005066536.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EKN05_RS14390 (EKN05_014400) 2768226..2768579 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EKN05_RS14400 (EKN05_014410) 2768958..2769269 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T120375 WP_001291435.1 NZ_CP037923:2764943-2765161 [Shigella flexneri 5a str. M90T]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T120375 NZ_CP037923:2764943-2765161 [Shigella flexneri 5a str. M90T]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT120375 WP_000344797.1 NZ_CP037923:2764543-2764917 [Shigella flexneri 5a str. M90T]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT120375 NZ_CP037923:2764543-2764917 [Shigella flexneri 5a str. M90T]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References