Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 197615..198269 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag EKN05_RS00985 Protein ID WP_000244767.1
Coordinates 197862..198269 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag EKN05_RS00980 Protein ID WP_000354044.1
Coordinates 197615..197881 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKN05_RS00955 (EKN05_000965) 192786..193529 + 744 Protein_188 SDR family oxidoreductase -
EKN05_RS00960 (EKN05_000970) 193586..195018 - 1433 Protein_189 6-phospho-beta-glucosidase BglA -
EKN05_RS00965 (EKN05_000975) 195063..195373 + 311 Protein_190 N(4)-acetylcytidine aminohydrolase -
EKN05_RS00970 (EKN05_000980) 195537..196196 + 660 WP_000250267.1 hemolysin III family protein -
EKN05_RS00975 (EKN05_000985) 196392..197372 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EKN05_RS00980 (EKN05_000990) 197615..197881 + 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
EKN05_RS00985 (EKN05_000995) 197862..198269 + 408 WP_000244767.1 protein YgfX Toxin
EKN05_RS00990 (EKN05_001000) 198309..198830 - 522 WP_001055867.1 flavodoxin FldB -
EKN05_RS00995 (EKN05_001005) 198942..199838 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EKN05_RS01000 (EKN05_001010) 199863..200573 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EKN05_RS01005 (EKN05_001015) 200579..202312 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T120359 WP_000244767.1 NZ_CP037923:197862-198269 [Shigella flexneri 5a str. M90T]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T120359 NZ_CP037923:197862-198269 [Shigella flexneri 5a str. M90T]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT120359 WP_000354044.1 NZ_CP037923:197615-197881 [Shigella flexneri 5a str. M90T]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT120359 NZ_CP037923:197615-197881 [Shigella flexneri 5a str. M90T]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References