Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1089806..1090426 Replicon chromosome
Accession NZ_CP037919
Organism Enterobacter cloacae complex sp. strain N13-01531

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag E2E36_RS05125 Protein ID WP_008499287.1
Coordinates 1089806..1090024 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag E2E36_RS05130 Protein ID WP_008499288.1
Coordinates 1090052..1090426 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2E36_RS05095 1085822..1086082 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
E2E36_RS05100 1086085..1086225 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
E2E36_RS05105 1086222..1086932 - 711 WP_133293898.1 GNAT family N-acetyltransferase -
E2E36_RS05110 1087033..1088493 + 1461 WP_133293899.1 PLP-dependent aminotransferase family protein -
E2E36_RS05115 1088465..1088932 - 468 WP_023310598.1 hypothetical protein -
E2E36_RS05120 1089050..1089601 - 552 WP_133293900.1 maltose O-acetyltransferase -
E2E36_RS05125 1089806..1090024 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
E2E36_RS05130 1090052..1090426 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
E2E36_RS05135 1090937..1094083 - 3147 WP_133293901.1 multidrug efflux RND transporter permease subunit AcrB -
E2E36_RS05140 1094106..1095299 - 1194 WP_045909753.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T120347 WP_008499287.1 NZ_CP037919:c1090024-1089806 [Enterobacter cloacae complex sp.]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T120347 NZ_CP037919:c1090024-1089806 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCACTCACAAAGATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TAACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT120347 WP_008499288.1 NZ_CP037919:c1090426-1090052 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT120347 NZ_CP037919:c1090426-1090052 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATCGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References