Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55531..56056 Replicon plasmid pYSP8-1-CTX-M-14
Accession NZ_CP037912
Organism Escherichia coli strain YSP8-1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E1J56_RS24900 Protein ID WP_001159868.1
Coordinates 55751..56056 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E1J56_RS24895 Protein ID WP_000813634.1
Coordinates 55531..55749 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1J56_RS24865 50923..51339 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
E1J56_RS24870 51336..51566 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
E1J56_RS24875 51831..52331 + 501 WP_000528931.1 hypothetical protein -
E1J56_RS24880 52344..53117 + 774 WP_000905949.1 hypothetical protein -
E1J56_RS24885 53284..54417 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
E1J56_RS24890 54451..54963 - 513 WP_000151784.1 hypothetical protein -
E1J56_RS24895 55531..55749 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E1J56_RS24900 55751..56056 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E1J56_RS24905 56057..56863 + 807 WP_000016982.1 site-specific integrase -
E1J56_RS24910 57637..58392 + 756 WP_000852146.1 replication initiation protein RepE -
E1J56_RS24915 58980..60146 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / erm(42) / ant(3'')-Ia / sitABCD / blaCTX-M-14 / qacE / cmlA1 / tet(M) iucA / iucB / iucC / iucD / iutA 1..149304 149304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T120337 WP_001159868.1 NZ_CP037912:55751-56056 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T120337 NZ_CP037912:55751-56056 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT120337 WP_000813634.1 NZ_CP037912:55531-55749 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT120337 NZ_CP037912:55531-55749 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References