Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 50923..51566 Replicon plasmid pYSP8-1-CTX-M-14
Accession NZ_CP037912
Organism Escherichia coli strain YSP8-1

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag E1J56_RS24865 Protein ID WP_001034046.1
Coordinates 50923..51339 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag E1J56_RS24870 Protein ID WP_001261278.1
Coordinates 51336..51566 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1J56_RS24850 46094..46510 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
E1J56_RS24855 46507..46737 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
E1J56_RS24860 47084..50878 + 3795 WP_001144732.1 hypothetical protein -
E1J56_RS24865 50923..51339 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
E1J56_RS24870 51336..51566 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E1J56_RS24875 51831..52331 + 501 WP_000528931.1 hypothetical protein -
E1J56_RS24880 52344..53117 + 774 WP_000905949.1 hypothetical protein -
E1J56_RS24885 53284..54417 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
E1J56_RS24890 54451..54963 - 513 WP_000151784.1 hypothetical protein -
E1J56_RS24895 55531..55749 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
E1J56_RS24900 55751..56056 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / erm(42) / ant(3'')-Ia / sitABCD / blaCTX-M-14 / qacE / cmlA1 / tet(M) iucA / iucB / iucC / iucD / iutA 1..149304 149304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T120336 WP_001034046.1 NZ_CP037912:c51339-50923 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T120336 NZ_CP037912:c51339-50923 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT120336 WP_001261278.1 NZ_CP037912:c51566-51336 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT120336 NZ_CP037912:c51566-51336 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References