Detailed information of TA system TA120334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 17274..17833
• Replicon: plasmid pYSP8-1-CTX-M-14
• Accession: NZ_CP037912
• Organism: Escherichia coli strain YSP8-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: E1J56_RS24680
• Protein ID: WP_000421272.1
• Coordinates: 17558..17833 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: E1J56_RS24675
• Protein ID: WP_001387467.1
• Coordinates: 17274..17558 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J56_RS24645	12392..13183	-	792	WP_032491824.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA22	-
E1J56_RS24650	13332..14345	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
E1J56_RS25690	14548..14784	+	237	Protein_15	DUF3330 domain-containing protein	-
E1J56_RS24660	14837..15541	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
E1J56_RS24665	15602..15871	+	270	Protein_17	2-keto-3-deoxygluconate permease	-
E1J56_RS24670	15917..16669	-	753	WP_000562172.1	IclR family transcriptional regulator	-
E1J56_RS24675	17274..17558	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
E1J56_RS24680	17558..17833	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E1J56_RS24685	17939..18232	+	294	WP_001105060.1	hypothetical protein	-
E1J56_RS24690	18267..18395	-	129	Protein_22	IS481 family transposase	-
E1J56_RS24695	18428..19598	-	1171	Protein_23	hypothetical protein	-
E1J56_RS24710	21427..21734	+	308	Protein_25	type II toxin-antitoxin system ParD family antitoxin	-
E1J56_RS24720	22077..22259	+	183	WP_042344623.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / erm(42) / ant(3'')-Ia / sitABCD / blaCTX-M-14 / qacE / cmlA1 / tet(M)	iucA / iucB / iucC / iucD / iutA	1..149304	149304
	-	flank	IS/Tn	ant(3'')-Ia	-	11847..13297	1450

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T120334 WP_000421272.1 NZ_CP037912:17558-17833 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T120334 NZ_CP037912:17558-17833 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT120334 WP_001387467.1 NZ_CP037912:17274-17558 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT120334 NZ_CP037912:17274-17558 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880