120333

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 112020..112485 Replicon plasmid pYSP8-1
Accession NZ_CP037911
Organism Escherichia coli strain YSP8-1

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag E1J56_RS23765 Protein ID WP_001230707.1
Coordinates 112234..112485 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag E1J56_RS23760 Protein ID WP_000801440.1
Coordinates 112020..112247 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1J56_RS23710 107427..107642 - 216 WP_000760375.1 EexN family lipoprotein -
E1J56_RS23715 107639..107932 - 294 WP_000134733.1 LuxR family transcriptional regulator -
E1J56_RS23720 108009..109238 - 1230 WP_113396640.1 relaxase/mobilization nuclease domain-containing protein -
E1J56_RS23725 109242..109745 - 504 WP_000781814.1 hypothetical protein -
E1J56_RS23735 110105..110236 - 132 WP_001353745.1 toxin-antitoxin system HicB family antitoxin -
E1J56_RS23740 110539..110844 - 306 WP_015586054.1 hypothetical protein -
E1J56_RS23745 110841..111257 - 417 WP_012421208.1 tail-associated lysozyme -
E1J56_RS23750 111267..111566 - 300 WP_000650311.1 hypothetical protein -
E1J56_RS23760 112020..112247 - 228 WP_000801440.1 hypothetical protein Antitoxin
E1J56_RS23765 112234..112485 - 252 WP_001230707.1 hypothetical protein Toxin
E1J56_RS23770 112482..113024 - 543 WP_001083906.1 hypothetical protein -
E1J56_RS23775 113130..113645 - 516 WP_001025395.1 J domain-containing protein -
E1J56_RS23785 114285..114635 - 351 WP_000127482.1 addiction module antitoxin -
E1J56_RS23790 114632..114967 - 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -
E1J56_RS23795 116222..117088 + 867 WP_001353743.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(X4) / erm(B) / floR / qnrS1 / blaTEM-1B / ant(3'')-Ia / lnu(G) - 1..239511 239511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-118)

Antitoxin

SpoVT-AbrB domain

(18-55)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T120333 WP_001230707.1 NZ_CP037911:c112485-112234 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T120333 NZ_CP037911:c112485-112234 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT120333 WP_000801440.1 NZ_CP037911:c112247-112020 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT120333 NZ_CP037911:c112247-112020 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References