Detailed information of TA system TA120331

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4233684..4234519
• Replicon: chromosome
• Accession: NZ_CP037910
• Organism: Escherichia coli strain YSP8-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: E1J56_RS20865
• Protein ID: WP_057109034.1
• Coordinates: 4234142..4234519 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1E5MC43
• Locus tag: E1J56_RS20860
• Protein ID: WP_063112944.1
• Coordinates: 4233684..4234052 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J56_RS20820	4229036..4229716	+	681	WP_001282919.1	WYL domain-containing protein	-
E1J56_RS20825	4229864..4230541	+	678	WP_063073422.1	hypothetical protein	-
E1J56_RS20830	4230547..4230699	+	153	WP_001696589.1	DUF905 family protein	-
E1J56_RS20835	4230800..4231618	+	819	WP_096211214.1	DUF945 domain-containing protein	-
E1J56_RS20840	4231710..4232195	+	486	WP_000213716.1	antirestriction protein	-
E1J56_RS20845	4232211..4232687	+	477	WP_001186780.1	RadC family protein	-
E1J56_RS20850	4232750..4232971	+	222	WP_000692323.1	DUF987 domain-containing protein	-
E1J56_RS20855	4232990..4233634	+	645	WP_045146861.1	hypothetical protein	-
E1J56_RS20860	4233684..4234052	+	369	WP_063112944.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E1J56_RS20865	4234142..4234519	+	378	WP_057109034.1	TA system toxin CbtA family protein	Toxin
E1J56_RS20870	4234516..4234665	+	150	Protein_4056	hypothetical protein	-
E1J56_RS20875	4234741..4234938	+	198	WP_023563519.1	DUF957 domain-containing protein	-
E1J56_RS20880	4235023..4235865	+	843	WP_001529559.1	DUF4942 domain-containing protein	-
E1J56_RS20885	4236614..4238152	+	1539	WP_001751945.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4209992..4245965	35973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13934.86 Da
• Isoelectric Point: 6.8517

>T120331 WP_057109034.1 NZ_CP037910:4234142-4234519 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T120331 NZ_CP037910:4234142-4234519 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13724.52 Da
• Isoelectric Point: 6.8270

>AT120331 WP_063112944.1 NZ_CP037910:4233684-4234052 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT120331 NZ_CP037910:4233684-4234052 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCACGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E5MC43