Detailed information of TA system TA120329

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3634695..3635529
• Replicon: chromosome
• Accession: NZ_CP037910
• Organism: Escherichia coli strain YSP8-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X7CAN1
• Locus tag: E1J56_RS17825
• Protein ID: WP_039025883.1
• Coordinates: 3635152..3635529 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2X5LBZ2
• Locus tag: E1J56_RS17820
• Protein ID: WP_039025882.1
• Coordinates: 3634695..3635063 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J56_RS17795	3631811..3632629	+	819	WP_001234702.1	DUF945 domain-containing protein	-
E1J56_RS17800	3632720..3633205	+	486	WP_000214316.1	antirestriction protein	-
E1J56_RS17805	3633220..3633696	+	477	WP_001186742.1	RadC family protein	-
E1J56_RS17810	3633765..3633986	+	222	WP_000692312.1	DUF987 domain-containing protein	-
E1J56_RS17815	3634001..3634645	+	645	WP_039025881.1	hypothetical protein	-
E1J56_RS17820	3634695..3635063	+	369	WP_039025882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E1J56_RS17825	3635152..3635529	+	378	WP_039025883.1	type IV toxin-antitoxin system toxin CbtA	Toxin
E1J56_RS17830	3635526..3636014	+	489	Protein_3472	hypothetical protein	-
E1J56_RS17835	3636034..3636231	+	198	WP_000772024.1	DUF957 domain-containing protein	-
E1J56_RS17840	3636328..3637176	+	849	WP_039025884.1	DUF4942 domain-containing protein	-
E1J56_RS17845	3637269..3638588	-	1320	WP_000144688.1	site-specific integrase	-
E1J56_RS17850	3638814..3639977	-	1164	WP_000051887.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3606713..3661956	55243

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14176.22 Da
• Isoelectric Point: 7.8523

>T120329 WP_039025883.1 NZ_CP037910:3635152-3635529 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T120329 NZ_CP037910:3635152-3635529 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13792.52 Da
• Isoelectric Point: 5.9582

>AT120329 WP_039025882.1 NZ_CP037910:3634695-3635063 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT120329 NZ_CP037910:3634695-3635063 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A2X7CAN1

Antitoxin

• AlphaFold DB: A0A2X5LBZ2