Detailed information of TA system TA120325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2245897..2246423
• Replicon: chromosome
• Accession: NZ_CP037910
• Organism: Escherichia coli strain YSP8-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: E1J56_RS10885
• Protein ID: WP_000323025.1
• Coordinates: 2246136..2246423 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: E1J56_RS10880
• Protein ID: WP_000534858.1
• Coordinates: 2245897..2246136 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J56_RS10850	2241259..2241615	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
E1J56_RS25735	2242226..2242513	+	288	Protein_2117	hypothetical protein	-
E1J56_RS10865	2243282..2244595	+	1314	WP_021553632.1	ISNCY family transposase	-
E1J56_RS10870	2245032..2245364	-	333	WP_001301033.1	protein FlxA	-
E1J56_RS10875	2245567..2245872	-	306	WP_001326990.1	hypothetical protein	-
E1J56_RS10880	2245897..2246136	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
E1J56_RS10885	2246136..2246423	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
E1J56_RS10890	2246495..2246650	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
E1J56_RS10895	2246867..2247118	+	252	WP_000980994.1	hypothetical protein	-
E1J56_RS10900	2247185..2247463	+	279	WP_012304870.1	hypothetical protein	-
E1J56_RS10905	2247465..2248514	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
E1J56_RS10910	2248528..2249280	+	753	WP_001047135.1	antitermination protein	-
E1J56_RS10915	2249558..2249647	-	90	WP_122985911.1	hypothetical protein	-
E1J56_RS10920	2249702..2249914	-	213	WP_024172454.1	cold shock-like protein CspF	-
E1J56_RS10925	2250213..2250428	+	216	WP_000066485.1	cold shock-like protein CspB	-
E1J56_RS25885	2250792..2250962	-	171	WP_001405948.1	putative zinc-binding protein YnfU	-
E1J56_RS10930	2251181..2251396	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2211415..2299785	88370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T120325 WP_000323025.1 NZ_CP037910:2246136-2246423 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T120325 NZ_CP037910:2246136-2246423 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT120325 WP_000534858.1 NZ_CP037910:2245897-2246136 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT120325 NZ_CP037910:2245897-2246136 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8