Detailed information of TA system TA120313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 805297..805895
• Replicon: chromosome
• Accession: NZ_CP037910
• Organism: Escherichia coli strain YSP8-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: E1J56_RS03940
• Protein ID: WP_053291281.1
• Coordinates: 805518..805895 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: E1J56_RS03935
• Protein ID: WP_001603498.1
• Coordinates: 805297..805518 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J56_RS03920	800897..801577	+	681	WP_053270475.1	fimbrial assembly chaperone	-
E1J56_RS03925	801595..804081	+	2487	WP_111970038.1	fimbria/pilus outer membrane usher protein	-
E1J56_RS03930	804093..805112	+	1020	WP_136655365.1	type 1 fimbrial protein	-
E1J56_RS03935	805297..805518	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E1J56_RS03940	805518..805895	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
E1J56_RS03950	806005..806220	-	216	Protein_773	transposase	-
E1J56_RS03955	806406..807668	-	1263	WP_136655366.1	integrase arm-type DNA-binding domain-containing protein	-
E1J56_RS03965	808047..808754	-	708	WP_000234514.1	DUF554 domain-containing protein	-
E1J56_RS25855	808907..808990	+	84	WP_211180506.1	protein YqgH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	788934..813874	24940
	-	flank	IS/Tn	-	-	805939..806220	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T120313 WP_053291281.1 NZ_CP037910:805518-805895 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T120313 NZ_CP037910:805518-805895 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT120313 WP_001603498.1 NZ_CP037910:805297-805518 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT120313 NZ_CP037910:805297-805518 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0