120308

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 22929..23394 Replicon plasmid pLHM10-1-MCR-1
Accession NZ_CP037906
Organism Escherichia coli strain LHM10-1

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag E1J55_RS24730 Protein ID WP_001230707.1
Coordinates 23143..23394 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag E1J55_RS24725 Protein ID WP_000801440.1
Coordinates 22929..23156 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1J55_RS24675 18159..18374 - 216 WP_000760375.1 EexN family lipoprotein -
E1J55_RS24680 18371..18664 - 294 WP_060598542.1 LuxR family transcriptional regulator -
E1J55_RS24685 18741..19970 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
E1J55_RS24690 19974..20477 - 504 WP_000781813.1 hypothetical protein -
E1J55_RS24700 20838..21185 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
E1J55_RS24705 21182..21430 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
E1J55_RS24710 21735..22085 - 351 WP_000854259.1 hypothetical protein -
E1J55_RS24715 22176..22475 - 300 WP_000650309.1 hypothetical protein -
E1J55_RS24725 22929..23156 - 228 WP_000801440.1 hypothetical protein Antitoxin
E1J55_RS24730 23143..23394 - 252 WP_001230707.1 hypothetical protein Toxin
E1J55_RS24735 23391..23933 - 543 WP_001083906.1 hypothetical protein -
E1J55_RS24740 24038..24553 - 516 WP_001025395.1 J domain-containing protein -
E1J55_RS24750 25968..26834 + 867 WP_001353743.1 replication initiation protein -
E1J55_RS24755 26847..27251 + 405 WP_012421203.1 hypothetical protein -
E1J55_RS24760 27386..27913 + 528 WP_000577043.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33301 33301


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-76)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T120308 WP_001230707.1 NZ_CP037906:c23394-23143 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T120308 NZ_CP037906:c23394-23143 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT120308 WP_000801440.1 NZ_CP037906:c23156-22929 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT120308 NZ_CP037906:c23156-22929 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References