Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 20838..21430 Replicon plasmid pLHM10-1-MCR-1
Accession NZ_CP037906
Organism Escherichia coli strain LHM10-1

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag E1J55_RS24705 Protein ID WP_000520549.1
Coordinates 21182..21430 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag E1J55_RS24700 Protein ID WP_000681613.1
Coordinates 20838..21185 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1J55_RS24665 16843..17115 - 273 WP_001328551.1 TrbC/VirB2 family protein -
E1J55_RS24670 17183..17803 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
E1J55_RS24675 18159..18374 - 216 WP_000760375.1 EexN family lipoprotein -
E1J55_RS24680 18371..18664 - 294 WP_060598542.1 LuxR family transcriptional regulator -
E1J55_RS24685 18741..19970 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
E1J55_RS24690 19974..20477 - 504 WP_000781813.1 hypothetical protein -
E1J55_RS24700 20838..21185 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E1J55_RS24705 21182..21430 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
E1J55_RS24710 21735..22085 - 351 WP_000854259.1 hypothetical protein -
E1J55_RS24715 22176..22475 - 300 WP_000650309.1 hypothetical protein -
E1J55_RS24725 22929..23156 - 228 WP_000801440.1 hypothetical protein -
E1J55_RS24730 23143..23394 - 252 WP_001230707.1 hypothetical protein -
E1J55_RS24735 23391..23933 - 543 WP_001083906.1 hypothetical protein -
E1J55_RS24740 24038..24553 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33301 33301


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T120307 WP_000520549.1 NZ_CP037906:c21430-21182 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T120307 NZ_CP037906:c21430-21182 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT120307 WP_000681613.1 NZ_CP037906:c21185-20838 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT120307 NZ_CP037906:c21185-20838 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References