Detailed information of TA system TA120306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 9478..10079
• Replicon: plasmid unnamed2
• Accession: NZ_CP037905
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: E1J55_RS24075
• Protein ID: WP_001216045.1
• Coordinates: 9699..10079 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: E1J55_RS24070
• Protein ID: WP_001190712.1
• Coordinates: 9478..9699 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS24020	5020..5574	+	555	Protein_8	DUF551 domain-containing protein	-
E1J55_RS24025	5571..5831	+	261	WP_136698663.1	eaa protein	-
E1J55_RS24030	5908..6141	+	234	WP_000517420.1	hypothetical protein	-
E1J55_RS24035	6320..6613	+	294	WP_000268000.1	hypothetical protein	-
E1J55_RS24040	6620..6994	+	375	WP_000988658.1	hypothetical protein	-
E1J55_RS24045	6976..7620	+	645	WP_136698664.1	hypothetical protein	-
E1J55_RS24050	7617..7979	+	363	WP_001261544.1	hypothetical protein	-
E1J55_RS25415	8054..8191	-	138	WP_000123562.1	hypothetical protein	-
E1J55_RS24060	8641..8892	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
E1J55_RS24065	9016..9405	+	390	WP_000506726.1	DNA repair protein	-
E1J55_RS24070	9478..9699	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E1J55_RS24075	9699..10079	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
E1J55_RS24080	10084..10263	+	180	WP_000113019.1	hypothetical protein	-
E1J55_RS24085	10291..11334	+	1044	WP_000648824.1	DUF968 domain-containing protein	-
E1J55_RS24090	11423..11875	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
E1J55_RS24095	11962..13155	+	1194	WP_136698665.1	terminase	-
E1J55_RS24100	13155..14639	+	1485	WP_096321054.1	terminase	-
E1J55_RS24105	14861..14980	+	120	WP_071594056.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98254	98254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T120306 WP_001216045.1 NZ_CP037905:9699-10079 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T120306 NZ_CP037905:9699-10079 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT120306 WP_001190712.1 NZ_CP037905:9478-9699 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT120306 NZ_CP037905:9478-9699 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6