Detailed information of TA system TA120290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3541519..3542356
• Replicon: chromosome
• Accession: NZ_CP037903
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: E1J55_RS17210
• Protein ID: WP_000227784.1
• Coordinates: 3541814..3542356 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: E1J55_RS17205
• Protein ID: WP_001297137.1
• Coordinates: 3541519..3541830 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS17180	3536539..3537486	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
E1J55_RS17185	3537508..3539499	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
E1J55_RS17190	3539489..3540103	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
E1J55_RS17195	3540103..3540432	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
E1J55_RS17200	3540444..3541334	+	891	WP_000971336.1	heme o synthase	-
E1J55_RS17205	3541519..3541830	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
E1J55_RS17210	3541814..3542356	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
E1J55_RS17215	3542412..3543347	-	936	WP_136698416.1	sel1 repeat family protein	-
E1J55_RS17220	3543755..3545119	+	1365	WP_001000954.1	MFS transporter	-
E1J55_RS17225	3545247..3545738	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
E1J55_RS17230	3545906..3546817	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T120290 WP_000227784.1 NZ_CP037903:3541814-3542356 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T120290 NZ_CP037903:3541814-3542356 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT120290 WP_001297137.1 NZ_CP037903:3541519-3541830 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT120290 NZ_CP037903:3541519-3541830 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3