Detailed information of TA system TA120288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2444325..2444963
• Replicon: chromosome
• Accession: NZ_CP037903
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2X7GI71
• Locus tag: E1J55_RS11745
• Protein ID: WP_032231106.1
• Coordinates: 2444787..2444963 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E1J55_RS11740
• Protein ID: WP_001270286.1
• Coordinates: 2444325..2444741 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS11720	2439477..2440418	-	942	WP_001251331.1	ABC transporter permease	-
E1J55_RS11725	2440419..2441432	-	1014	WP_136698316.1	ABC transporter ATP-binding protein	-
E1J55_RS11730	2441450..2442595	-	1146	WP_000047425.1	ABC transporter substrate-binding protein	-
E1J55_RS11735	2442840..2444246	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
E1J55_RS11740	2444325..2444741	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
E1J55_RS11745	2444787..2444963	-	177	WP_032231106.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
E1J55_RS11750	2445185..2445415	+	231	WP_000494244.1	YncJ family protein	-
E1J55_RS11755	2445507..2447468	-	1962	WP_136698317.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
E1J55_RS11760	2447541..2448077	-	537	WP_000429151.1	DNA-binding transcriptional regulator SutR	-
E1J55_RS11765	2448169..2449205	+	1037	Protein_2287	benzoate/H(+) symporter BenE family transporter	-
E1J55_RS11770	2449240..2449908	-	669	WP_001261021.1	DUF3313 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6680.72 Da
• Isoelectric Point: 10.9223

>T120288 WP_032231106.1 NZ_CP037903:c2444963-2444787 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPCHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T120288 NZ_CP037903:c2444963-2444787 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGTGTCACCCTAGCGATGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT120288 WP_001270286.1 NZ_CP037903:c2444741-2444325 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT120288 NZ_CP037903:c2444741-2444325 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2X7GI71