Detailed information of TA system TA120273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 196317..196882
• Replicon: plasmid p1
• Accession: NZ_CP037902
• Organism: Cupriavidus metallidurans strain BS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q58AR3
• Locus tag: DDF84_RS32510
• Protein ID: WP_011229306.1
• Coordinates: 196317..196688 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q58AR4
• Locus tag: DDF84_RS32515
• Protein ID: WP_011229305.1
• Coordinates: 196685..196882 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDF84_RS32480	191838..192386	+	549	WP_017513745.1	hypothetical protein	-
DDF84_RS32485	192398..192946	-	549	WP_038316447.1	hypothetical protein	-
DDF84_RS32490	193011..193514	-	504	WP_111733969.1	hypothetical protein	-
DDF84_RS32495	193544..193876	-	333	WP_111733968.1	hypothetical protein	-
DDF84_RS33810	193997..195571	-	1575	WP_189443404.1	hypothetical protein	-
DDF84_RS32505	195709..195927	-	219	Protein_200	hypothetical protein	-
DDF84_RS32510	196317..196688	-	372	WP_011229306.1	type II toxin-antitoxin system VapC family toxin	Toxin
DDF84_RS32515	196685..196882	-	198	WP_011229305.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DDF84_RS32520	197048..197773	+	726	WP_011514795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
DDF84_RS32525	197770..198720	+	951	WP_011229304.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
DDF84_RS32530	198754..199023	+	270	WP_124682103.1	hypothetical protein	-
DDF84_RS32535	199312..199620	+	309	WP_017514069.1	hypothetical protein	-
DDF84_RS32540	200059..200349	+	291	WP_017514070.1	hypothetical protein	-
DDF84_RS32545	200393..200857	+	465	WP_017514071.1	IS66 family insertion sequence hypothetical protein	-
DDF84_RS32550	200857..201207	+	351	WP_017514072.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY	1..364407	364407

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13613.83 Da
• Isoelectric Point: 5.6319

>T120273 WP_011229306.1 NZ_CP037902:c196688-196317 [Cupriavidus metallidurans]
MILVDTSVWIDHLNASDPMLISLLTQERVLIHPYVIGEISLGSLRDRDVVLGALRDLPRAPVATPEETLYLIEREGLFNR
GIGYGDTSLLASARLQPGIAIWTRDKRLKKVADELCLGAVLSH

Nucleotide

• Length: 372 bp

>T120273 NZ_CP037902:c196688-196317 [Cupriavidus metallidurans]
ATGATTCTTGTCGACACGTCGGTCTGGATTGACCACCTCAACGCTTCTGATCCGATGCTGATCAGCCTCTTAACCCAGGA
GCGTGTGCTGATTCATCCCTATGTGATCGGTGAGATTTCGCTTGGTAGTCTGCGGGACCGAGATGTCGTGCTTGGCGCTC
TGCGCGATTTGCCGCGAGCGCCTGTCGCAACACCAGAGGAGACCCTGTATTTGATCGAGCGCGAAGGCTTGTTCAACCGT
GGAATCGGATATGGCGATACATCATTGTTGGCATCCGCGCGTCTCCAACCAGGCATCGCCATCTGGACGCGTGACAAGCG
ATTGAAGAAAGTTGCTGATGAACTCTGTCTCGGCGCGGTCCTTTCGCACTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7132.21 Da
• Isoelectric Point: 10.3509

>AT120273 WP_011229305.1 NZ_CP037902:c196882-196685 [Cupriavidus metallidurans]
MRTTIALDDELLAKAQAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIEGVPRRRQDTK

Nucleotide

• Length: 198 bp

>AT120273 NZ_CP037902:c196882-196685 [Cupriavidus metallidurans]
ATGCGTACGACCATTGCGCTTGACGATGAACTGTTGGCCAAGGCCCAAGCCTATACGGGGCTGGAAGAGAAAACGGCACT
TGTGCGCGAGGCGCTAAAGGCCTTGATCCAGCGTGAAGCCGCCAAACGACTCGCGAACCTTGGCGGGAGCCAACCGGGCA
TCGAAGGGGTACCGCGTCGCCGGCAGGACACCAAATGA

Structures

Toxin

• AlphaFold DB: A0A482J4V6

Antitoxin

• AlphaFold DB: A0A482J603