Detailed information of TA system TA120267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 14472..14997
• Replicon: plasmid PNCS014854 strain 4[5]12:i:-
• Accession: NZ_CP037873
• Organism: Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain PNCS014854

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: E1965_RS00090
• Protein ID: WP_001159863.1
• Coordinates: 14692..14997 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: E1965_RS00085
• Protein ID: WP_000813641.1
• Coordinates: 14472..14690 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1965_RS00060	9759..10140	+	382	Protein_11	YgiW/YdeI family stress tolerance OB fold protein	-
E1965_RS00065	10309..10753	+	445	Protein_12	site-specific integrase	-
E1965_RS00070	11012..12001	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
E1965_RS00075	12495..12791	-	297	WP_001687482.1	hypothetical protein	-
E1965_RS00080	13275..13796	-	522	WP_010999942.1	hypothetical protein	-
E1965_RS00085	14472..14690	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E1965_RS00090	14692..14997	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
E1965_RS00095	14999..15289	+	291	WP_001266176.1	hypothetical protein	-
E1965_RS00100	15286..15807	+	522	WP_000198608.1	hypothetical protein	-
E1965_RS00105	15842..16624	+	783	WP_000082169.1	site-specific integrase	-
E1965_RS00110	16633..17346	+	714	Protein_21	EAL domain-containing protein	-
E1965_RS00115	17350..17859	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
E1965_RS00120	17853..18338	+	486	WP_000905606.1	membrane protein	-
E1965_RS00125	18615..18902	-	288	WP_071530243.1	hypothetical protein	-
E1965_RS00130	19058..19618	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93844	93844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Cro/C1-type helix-turn-helix domain (46-89)

Antitoxin

• Toxin HigB-like (13-88)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T120267 WP_001159863.1 NZ_CP037873:14692-14997 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T120267 NZ_CP037873:14692-14997 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT120267 WP_000813641.1 NZ_CP037873:14472-14690 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT120267 NZ_CP037873:14472-14690 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6