Detailed information of TA system TA120265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1390015..1390616
• Replicon: chromosome
• Accession: NZ_CP037865
• Organism: Pasteurella multocida subsp. multocida strain HN02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: E0Z11_RS07095
• Protein ID: WP_139639116.1
• Coordinates: 1390015..1390329 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E0Z11_RS07100
• Protein ID: WP_005720732.1
• Coordinates: 1390326..1390616 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0Z11_RS07065	1385982..1386449	+	468	Protein_1337	CDP-alcohol phosphatidyltransferase family protein	-
E0Z11_RS12095	1386451..1386609	-	159	WP_176558171.1	hypothetical protein	-
E0Z11_RS07070	1386688..1386957	+	270	Protein_1339	hypothetical protein	-
E0Z11_RS07075	1387009..1387302	+	294	Protein_1340	Rha family transcriptional regulator	-
E0Z11_RS07080	1387402..1388058	-	657	WP_005719807.1	hypothetical protein	-
E0Z11_RS07085	1388383..1389180	-	798	WP_005719805.1	hypothetical protein	-
E0Z11_RS07090	1389357..1389584	-	228	WP_139617775.1	hypothetical protein	-
E0Z11_RS07095	1390015..1390329	+	315	WP_139639116.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E0Z11_RS07100	1390326..1390616	+	291	WP_005720732.1	putative addiction module antidote protein	Antitoxin
E0Z11_RS07105	1390637..1391524	-	888	WP_139639118.1	hypothetical protein	-
E0Z11_RS07110	1391517..1392047	-	531	WP_047918155.1	hypothetical protein	-
E0Z11_RS07115	1392147..1392836	-	690	WP_005720261.1	helix-turn-helix transcriptional regulator	-
E0Z11_RS07120	1392964..1393173	+	210	WP_005720263.1	helix-turn-helix transcriptional regulator	-
E0Z11_RS07125	1393379..1393783	-	405	WP_139639119.1	VOC family protein	-
E0Z11_RS07130	1393916..1394830	-	915	WP_139617779.1	c-type cytochrome biogenesis protein CcmI	-
E0Z11_RS07135	1394827..1395291	-	465	WP_005721339.1	cytochrome c-type biogenesis protein CcmH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1387009..1407427	20418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-91)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11717.59 Da
• Isoelectric Point: 9.7597

>T120265 WP_139639116.1 NZ_CP037865:1390015-1390329 [Pasteurella multocida subsp. multocida]
MLDVIETTVFNKWLKELKDLSAKAAILARINRAKNGNFGDHKSVGDGLYEMRIMKGAGYRVYYAQYKDVTYLLICGGDKS
TQKADIAKAKALWEEIKQKEGVNV

Nucleotide

• Length: 315 bp

>T120265 NZ_CP037865:1390015-1390329 [Pasteurella multocida subsp. multocida]
ATGCTCGATGTAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAAGATTTGTCGGCTAAGGCAGCTATTCT
TGCTCGTATTAATAGAGCTAAAAATGGTAATTTTGGCGATCATAAATCAGTTGGTGATGGTTTATATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATTGCTAAAGCAAAAGCGTTATGGGAAGAAATTAAGCAGAAAGAGGGGGTAAATGTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.35 Da
• Isoelectric Point: 4.8605

>AT120265 WP_005720732.1 NZ_CP037865:1390326-1390616 [Pasteurella multocida subsp. multocida]
MSKKVELKPFDIAEYLDSEEMIAAYLSEVLETGDTNEFISALGDVARARGMTELAEKTGLGRESLYKTLSYGSKPRFDTI
MKITQALGIKLVPMHI

Nucleotide

• Length: 291 bp

>AT120265 NZ_CP037865:1390326-1390616 [Pasteurella multocida subsp. multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATATCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAGTTTTAGAAACAGGTGATACTAATGAATTTATTTCTGCGCTAGGTGATGTTGCAAGAGCGAGAGGAATGACGGAAT
TAGCAGAAAAAACGGGGTTGGGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCCATGCACATCTAA