Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1292821..1293441 Replicon chromosome
Accession NZ_CP037864
Organism Citrobacter sp. LY-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag E1B03_RS07075 Protein ID WP_002892050.1
Coordinates 1292821..1293039 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag E1B03_RS07080 Protein ID WP_003021733.1
Coordinates 1293067..1293441 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1B03_RS07040 1288179..1288808 + 630 WP_103772083.1 hypothetical protein -
E1B03_RS07045 1288873..1289226 + 354 WP_003831033.1 DUF1428 family protein -
E1B03_RS07050 1289278..1290831 - 1554 WP_103772082.1 EAL domain-containing protein -
E1B03_RS07055 1291020..1291280 + 261 WP_038638331.1 type B 50S ribosomal protein L31 -
E1B03_RS07060 1291282..1291422 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
E1B03_RS07065 1291502..1291972 - 471 WP_016152071.1 YlaC family protein -
E1B03_RS07070 1292077..1292640 - 564 WP_103772081.1 maltose O-acetyltransferase -
E1B03_RS07075 1292821..1293039 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
E1B03_RS07080 1293067..1293441 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
E1B03_RS07085 1293932..1297081 - 3150 WP_103772079.1 efflux RND transporter permease AcrB -
E1B03_RS07090 1297104..1298297 - 1194 WP_133085926.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T120251 WP_002892050.1 NZ_CP037864:c1293039-1292821 [Citrobacter sp. LY-1]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T120251 NZ_CP037864:c1293039-1292821 [Citrobacter sp. LY-1]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT120251 WP_003021733.1 NZ_CP037864:c1293441-1293067 [Citrobacter sp. LY-1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT120251 NZ_CP037864:c1293441-1293067 [Citrobacter sp. LY-1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGTCT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References