Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 2943..3520 Replicon plasmid unnamed1
Accession NZ_CP037862
Organism Citrobacter sp. LY-1

Toxin (Protein)


Gene name parE Uniprot ID I3W245
Locus tag E1B03_RS00020 Protein ID WP_015059150.1
Coordinates 2943..3254 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID E3DB50
Locus tag E1B03_RS00025 Protein ID WP_014542884.1
Coordinates 3242..3520 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1B03_RS00005 320..643 + 324 WP_207949448.1 plasmid mobilization relaxosome protein MobC -
E1B03_RS26135 582..1343 + 762 Protein_1 relaxase/mobilization nuclease domain-containing protein -
E1B03_RS26140 1321..1818 + 498 WP_157944932.1 MbeB family mobilization protein -
E1B03_RS26145 1825..2040 + 216 WP_032634148.1 MbeD family mobilization/exclusion protein -
E1B03_RS00015 2144..2575 - 432 WP_133085517.1 hypothetical protein -
E1B03_RS00020 2943..3254 - 312 WP_015059150.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E1B03_RS00025 3242..3520 - 279 WP_014542884.1 CopG family ribbon-helix-helix protein Antitoxin
E1B03_RS26150 3894..4055 + 162 WP_157944930.1 hypothetical protein -
E1B03_RS00030 4136..4696 - 561 WP_105277857.1 helix-turn-helix domain-containing protein -
E1B03_RS00035 5119..5565 + 447 WP_105277859.1 hypothetical protein -
E1B03_RS00045 6667..7536 + 870 WP_105277862.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..15855 15855


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-96)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11840.74 Da        Isoelectric Point: 9.8620

>T120246 WP_015059150.1 NZ_CP037862:c3254-2943 [Citrobacter sp. LY-1]
MPQVIVSALAQRDLQRLQDFLKTKNRLAARKAGEVIIRAIQQLKTQPDMGRPVSLLPLEYQELVIGFGDSGYVMLYRHDR
ETDRIVILTVRHQKEAGYHGLQE

Download         Length: 312 bp

>T120246 NZ_CP037862:c3254-2943 [Citrobacter sp. LY-1]
ATGCCACAGGTAATTGTCTCCGCACTGGCGCAGCGGGATTTACAGCGCCTGCAGGACTTTCTCAAAACCAAAAACCGGCT
GGCGGCCAGAAAAGCCGGTGAAGTGATCATCCGGGCTATCCAGCAACTGAAAACGCAGCCGGACATGGGCCGTCCGGTTT
CTCTTCTCCCGCTGGAATACCAGGAACTGGTGATCGGATTTGGCGACAGTGGTTATGTGATGCTCTATCGCCACGACCGG
GAAACGGACCGGATAGTGATCCTGACAGTCCGGCACCAGAAAGAAGCCGGATATCACGGCCTGCAGGAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10721.74 Da        Isoelectric Point: 4.6216

>AT120246 WP_014542884.1 NZ_CP037862:c3520-3242 [Citrobacter sp. LY-1]
MSAATSIRLDDELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLSAEEVEAWIDTWG
TENEQDAPPCHR

Download         Length: 279 bp

>AT120246 NZ_CP037862:c3520-3242 [Citrobacter sp. LY-1]
ATGTCAGCAGCGACATCCATCAGGCTTGACGATGAACTCAAAGACCGCCTGAAAACTCTTGCCGATGACCGCCATCGCTC
TGCTCATGCTCTGATGCTGGAAGCGATCACCGAATACATCGACCGGGAAGAAAAGCGCAGCCAGTACCTGAGGGATGGAC
AGGCCGCATGGCAGCACTATCAGGAAACCGGCCTGCACCTGAGCGCCGAAGAGGTGGAAGCGTGGATAGATACCTGGGGA
ACTGAGAATGAGCAGGATGCTCCACCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P6WJV0

Antitoxin

Source ID Structure
AlphaFold DB E3DB50

References