Detailed information of TA system TA120241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 161653..162293
• Replicon: chromosome
• Accession: NZ_CP037861
• Organism: Pasteurella multocida subsp. multocida strain HN01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V4NDJ2
• Locus tag: E0L18_RS00805
• Protein ID: WP_016533221.1
• Coordinates: 161653..161829 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V4Q272
• Locus tag: E0L18_RS00810
• Protein ID: WP_016533220.1
• Coordinates: 161877..162293 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0L18_RS00785	157036..158571	-	1536	WP_139616929.1	phage portal protein	-
E0L18_RS00790	158568..158789	-	222	WP_139616930.1	hypothetical protein	-
E0L18_RS00795	158786..160894	-	2109	WP_005719701.1	phage terminase large subunit family protein	-
E0L18_RS00800	160897..161373	-	477	WP_139616931.1	DUF1441 family protein	-
E0L18_RS00805	161653..161829	+	177	WP_016533221.1	type II toxin-antitoxin system HicA family toxin	Toxin
E0L18_RS00810	161877..162293	+	417	WP_016533220.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E0L18_RS00820	162518..162841	-	324	WP_014667795.1	DUF2570 family protein	-
E0L18_RS00825	162814..163344	-	531	WP_014667794.1	lysozyme	-
E0L18_RS00830	163341..163601	-	261	WP_014391475.1	hypothetical protein	-
E0L18_RS00835	163718..164275	+	558	WP_064964938.1	hypothetical protein	-
E0L18_RS00840	164402..164767	-	366	WP_139616933.1	hypothetical protein	-
E0L18_RS00845	164767..165369	-	603	WP_139616934.1	recombination protein NinG	-
E0L18_RS00850	165457..165669	-	213	WP_139616935.1	hypothetical protein	-
E0L18_RS00855	165743..166168	-	426	WP_139616936.1	recombination protein NinB	-
E0L18_RS00860	166171..166710	-	540	WP_139616937.1	DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	134964..205109	70145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-78)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6530.60 Da
• Isoelectric Point: 10.7333

>T120241 WP_016533221.1 NZ_CP037861:161653-161829 [Pasteurella multocida subsp. multocida]
MKQSEFLRWLKAQGVETKEGSNHIKLYLNGNQSALPRHPSKEIAKGTEIAVKKQLGLK

Nucleotide

• Length: 177 bp

>T120241 NZ_CP037861:161653-161829 [Pasteurella multocida subsp. multocida]
ATGAAACAAAGTGAGTTTTTAAGATGGCTGAAAGCTCAAGGGGTAGAAACTAAAGAAGGCTCAAACCACATTAAGCTCTA
CCTAAACGGCAACCAATCAGCTCTCCCAAGACATCCAAGCAAAGAGATAGCCAAGGGAACTGAAATAGCAGTTAAAAAGC
AATTAGGTCTAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15580.82 Da
• Isoelectric Point: 4.3715

>AT120241 WP_016533220.1 NZ_CP037861:161877-162293 [Pasteurella multocida subsp. multocida]
MLYPAKFDKEDNGLYAVSFRDIPEALTCGDNFDDAVAMAKDALVTSMDFYFEDFRKVPLPTEPEQDEVLIELPDSVFAKV
LLLNEMVEQNISNAELARRINVRPQEMQRITNISHSTKIDTISRALSALGKKLQLSVV

Nucleotide

• Length: 417 bp

>AT120241 NZ_CP037861:161877-162293 [Pasteurella multocida subsp. multocida]
ATGTTATATCCAGCAAAATTTGATAAAGAAGATAATGGGCTTTATGCTGTATCATTTCGTGATATACCTGAAGCCTTAAC
TTGTGGTGATAACTTCGACGATGCCGTCGCAATGGCAAAAGATGCCTTGGTTACATCAATGGATTTTTACTTTGAGGACT
TCCGCAAAGTGCCATTACCAACCGAGCCAGAGCAAGATGAGGTATTAATCGAGTTACCAGATAGTGTTTTTGCTAAAGTG
CTTTTATTAAATGAGATGGTTGAACAAAATATCTCTAATGCTGAATTAGCAAGAAGAATTAATGTTCGACCACAGGAAAT
GCAACGCATTACTAATATCTCGCATAGCACAAAAATCGACACAATAAGCCGTGCTTTGTCAGCACTCGGTAAAAAACTAC
AACTTTCAGTCGTCTAA

Structures

Toxin

• AlphaFold DB: V4NDJ2

Antitoxin

• AlphaFold DB: V4Q272