Detailed information of TA system TA120235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4523040..4523854
• Replicon: chromosome
• Accession: NZ_CP037857
• Organism: Escherichia coli BW25113 strain AM187

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: E1A98_RS21975
• Protein ID: WP_001054376.1
• Coordinates: 4523597..4523854 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: E1A98_RS21970
• Protein ID: WP_001309181.1
• Coordinates: 4523040..4523585 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1A98_RS21945	4518731..4520044	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
E1A98_RS21950	4520056..4520334	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
E1A98_RS21955	4520331..4521452	-	1122	WP_000010829.1	M42 family metallopeptidase	-
E1A98_RS22770	4521697..4521813	-	117	Protein_4259	VOC family protein	-
E1A98_RS21960	4521851..4522069	-	219	Protein_4260	hypothetical protein	-
E1A98_RS21965	4522238..4522984	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
E1A98_RS21970	4523040..4523585	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
E1A98_RS21975	4523597..4523854	-	258	WP_001054376.1	hypothetical protein	Toxin
E1A98_RS21980	4524231..4524476	-	246	Protein_4264	GNAT family N-acetyltransferase	-
E1A98_RS21985	4524592..4525832	+	1241	Protein_4265	helicase YjhR	-
E1A98_RS21990	4526415..4527395	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
E1A98_RS21995	4527460..4528566	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	4523040..4539880	16840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T120235 WP_001054376.1 NZ_CP037857:c4523854-4523597 [Escherichia coli BW25113]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T120235 NZ_CP037857:c4523854-4523597 [Escherichia coli BW25113]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT120235 WP_001309181.1 NZ_CP037857:c4523585-4523040 [Escherichia coli BW25113]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT120235 NZ_CP037857:c4523585-4523040 [Escherichia coli BW25113]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8