Detailed information of TA system TA120222

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2070255..2071086
• Replicon: chromosome
• Accession: NZ_CP037857
• Organism: Escherichia coli BW25113 strain AM187

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: E1A98_RS10335
• Protein ID: WP_000854814.1
• Coordinates: 2070712..2071086 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: E1A98_RS10330
• Protein ID: WP_001285584.1
• Coordinates: 2070255..2070623 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1A98_RS10315	2067922..2069454	+	1533	WP_001350525.1	hypothetical protein	-
E1A98_RS10320	2069451..2069897	+	447	WP_000187523.1	RadC family protein	-
E1A98_RS10325	2069960..2070181	+	222	WP_000692323.1	DUF987 domain-containing protein	-
E1A98_RS10330	2070255..2070623	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
E1A98_RS10335	2070712..2071086	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
E1A98_RS10340	2071083..2071277	+	195	WP_000988600.1	hypothetical protein	-
E1A98_RS22520	2071290..2071403	+	114	WP_001161660.1	DUF957 domain-containing protein	-
E1A98_RS10350	2071692..2071820	-	129	Protein_2010	transposase domain-containing protein	-
E1A98_RS10355	2071892..2072074	+	183	WP_001016348.1	hypothetical protein	-
E1A98_RS10360	2072175..2072504	-	330	WP_000450409.1	DUF496 family protein	-
E1A98_RS10365	2072676..2073734	-	1059	WP_001200891.1	FUSC family protein	-
E1A98_RS10370	2073932..2074405	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
E1A98_RS10375	2074524..2075690	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T120222 WP_000854814.1 NZ_CP037857:2070712-2071086 [Escherichia coli BW25113]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T120222 NZ_CP037857:2070712-2071086 [Escherichia coli BW25113]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT120222 WP_001285584.1 NZ_CP037857:2070255-2070623 [Escherichia coli BW25113]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT120222 NZ_CP037857:2070255-2070623 [Escherichia coli BW25113]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6