Detailed information of TA system TA120199

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 260044..260723
• Replicon: chromosome
• Accession: NZ_CP037857
• Organism: Escherichia coli BW25113 strain AM187

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: E1A98_RS01230
• Protein ID: WP_000854672.1
• Coordinates: 260044..260385 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: E1A98_RS01235
• Protein ID: WP_000070395.1
• Coordinates: 260406..260723 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1A98_RS01210	256099..257202	+	1104	WP_001285288.1	glutamate 5-kinase	-
E1A98_RS01215	257214..258467	+	1254	WP_133279790.1	glutamate-5-semialdehyde dehydrogenase	-
E1A98_RS01220	258753..259469	+	717	WP_133279792.1	hypothetical protein	-
E1A98_RS01230	260044..260385	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
E1A98_RS01235	260406..260723	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
E1A98_RS01240	260742..260963	-	222	WP_000691994.1	DUF987 domain-containing protein	-
E1A98_RS01245	260972..261448	-	477	WP_000811693.1	RadC family protein	-
E1A98_RS01250	261464..261922	-	459	WP_000211838.1	antirestriction protein	-
E1A98_RS01255	262020..262259	-	240	WP_000194654.1	DUF905 domain-containing protein	-
E1A98_RS01260	262336..262803	-	468	WP_001547765.1	hypothetical protein	-
E1A98_RS01265	262826..263269	-	444	WP_000824223.1	hypothetical protein	-
E1A98_RS01270	263269..263496	-	228	WP_001548158.1	hypothetical protein	-
E1A98_RS01275	263492..263683	-	192	Protein_248	DeoR family transcriptional regulator	-
E1A98_RS01280	263900..264721	-	822	WP_000197389.1	DUF945 domain-containing protein	-
E1A98_RS01285	264813..265676	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T120199 WP_000854672.1 NZ_CP037857:c260385-260044 [Escherichia coli BW25113]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T120199 NZ_CP037857:c260385-260044 [Escherichia coli BW25113]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT120199 WP_000070395.1 NZ_CP037857:c260723-260406 [Escherichia coli BW25113]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT120199 NZ_CP037857:c260723-260406 [Escherichia coli BW25113]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684