Detailed information of TA system TA120015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 509933..510374
• Replicon: chromosome
• Accession: NZ_CP037759
• Organism: Burkholderia pseudomallei strain BPs116

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: EYA88_RS02705
• Protein ID: WP_009939402.1
• Coordinates: 510201..510374 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: EYA88_RS02700
• Protein ID: WP_004525420.1
• Coordinates: 509933..510217 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYA88_RS02670	506310..506630	+	321	WP_174768775.1	DUF4212 domain-containing protein	-
EYA88_RS02675	506624..508639	+	2016	WP_004525415.1	cation acetate symporter	-
EYA88_RS02685	508880..509287	-	408	Protein_422	DDE-type integrase/transposase/recombinase	-
EYA88_RS02700	509933..510217	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
EYA88_RS02705	510201..510374	-	174	WP_009939402.1	BrnT family toxin	Toxin
EYA88_RS02710	511042..511317	+	276	WP_004552388.1	bacteriophage protein Gp49	-
EYA88_RS02715	511327..511458	+	132	WP_009923452.1	bacteriophage protein Gp48	-
EYA88_RS35985	511648..511866	+	219	WP_165940694.1	hypothetical protein	-
EYA88_RS02735	512165..512314	+	150	WP_004537646.1	bacteriophage protein Gp44	-
EYA88_RS02740	512311..513084	+	774	Protein_429	prohibitin family protein	-
EYA88_RS36800	513082..513486	+	405	Protein_430	DDE-type integrase/transposase/recombinase	-
EYA88_RS02745	513449..513877	+	429	Protein_431	IS110 family transposase	-
EYA88_RS02750	514104..514220	-	117	Protein_432	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	506624..515927	9303
	-	inside	IScluster/Tn	-	-	508880..519570	10690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T120015 WP_009939402.1 NZ_CP037759:c510374-510201 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T120015 NZ_CP037759:c510374-510201 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT120015 WP_004525420.1 NZ_CP037759:c510217-509933 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT120015 NZ_CP037759:c510217-509933 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8