Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17449..18092 Replicon plasmid pDHQP1701672_amr
Accession NZ_CP037744
Organism Klebsiella pneumoniae strain ST23

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag E1A84_RS27590 Protein ID WP_001044770.1
Coordinates 17676..18092 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag E1A84_RS27585 Protein ID WP_001261282.1
Coordinates 17449..17679 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1A84_RS27540 12647..13120 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
E1A84_RS27545 13212..13442 + 231 WP_011977773.1 hypothetical protein -
E1A84_RS27550 14334..15116 - 783 WP_004152340.1 site-specific integrase -
E1A84_RS27555 15116..15448 - 333 WP_004152339.1 hypothetical protein -
E1A84_RS27560 15455..15853 - 399 WP_004171440.1 hypothetical protein -
E1A84_RS27565 15879..16208 - 330 WP_004152337.1 hypothetical protein -
E1A84_RS27570 16236..16544 - 309 WP_004152336.1 hypothetical protein -
E1A84_RS27580 17031..17492 - 462 WP_072093212.1 hypothetical protein -
E1A84_RS27585 17449..17679 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E1A84_RS27590 17676..18092 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
E1A84_RS27595 18166..18678 + 513 Protein_19 AAA family ATPase -
E1A84_RS27600 18734..19431 + 698 Protein_20 IS1 family transposase -
E1A84_RS27605 19447..19557 + 111 Protein_21 mercuric transport protein periplasmic component -
E1A84_RS27610 19593..20015 + 423 WP_001340589.1 organomercurial transporter MerC -
E1A84_RS27615 20067..21761 + 1695 WP_000105636.1 mercury(II) reductase -
E1A84_RS27620 21779..22141 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
E1A84_RS27625 22138..22374 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
E1A84_RS27630 22410..23078 + 669 WP_001300294.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1A / blaOXA-9 / blaKPC-2 - 1..121239 121239
- flank IS/Tn - - 11259..12527 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T120001 WP_001044770.1 NZ_CP037744:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T120001 NZ_CP037744:17676-18092 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT120001 WP_001261282.1 NZ_CP037744:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT120001 NZ_CP037744:17449-17679 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References