Detailed information of TA system TA120000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 153607..154277
• Replicon: plasmid pDHQP1701672_hv
• Accession: NZ_CP037743
• Organism: Klebsiella pneumoniae strain ST23

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: E1A84_RS27070
• Protein ID: WP_004213072.1
• Coordinates: 153607..154050 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: E1A84_RS27075
• Protein ID: WP_004213073.1
• Coordinates: 154047..154277 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1A84_RS27025	149018..149293	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
E1A84_RS27030	149356..149847	+	492	WP_011251327.1	DM13 domain-containing protein	-
E1A84_RS27035	149896..150816	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
E1A84_RS27045	150907..151313	+	407	Protein_159	GAF domain-containing protein	-
E1A84_RS29170	151828..152463	-	636	Protein_160	response regulator	-
E1A84_RS27060	152880..153173	+	294	Protein_161	transposase	-
E1A84_RS27065	153177..153593	+	417	Protein_162	restriction endonuclease	-
E1A84_RS27070	153607..154050	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
E1A84_RS27075	154047..154277	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E1A84_RS27080	154885..156018	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
E1A84_RS27085	156034..156327	+	294	WP_004213076.1	hypothetical protein	-
E1A84_RS27090	156317..156523	-	207	WP_004213077.1	hypothetical protein	-
E1A84_RS27095	156875..157165	+	291	WP_004213078.1	hypothetical protein	-
E1A84_RS27100	157155..158054	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..227239	227239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T120000 WP_004213072.1 NZ_CP037743:c154050-153607 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T120000 NZ_CP037743:c154050-153607 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT120000 WP_004213073.1 NZ_CP037743:c154277-154047 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT120000 NZ_CP037743:c154277-154047 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620