Detailed information of TA system TA119971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4134695..4135166
• Replicon: chromosome
• Accession: NZ_CP037449
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E0708_RS19420
• Protein ID: WP_001303511.1
• Coordinates: 4134695..4134973 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E0708_RS19425
• Protein ID: WP_001302048.1
• Coordinates: 4134975..4135166 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0708_RS19385	4129908..4130176	-	269	Protein_3776	excisionase	-
E0708_RS19390	4130238..4132676	-	2439	WP_000102132.1	exonuclease	-
E0708_RS19395	4132769..4132957	-	189	WP_000199475.1	DUF1482 family protein	-
E0708_RS19400	4132954..4133142	-	189	WP_000449175.1	cell division inhibitor	-
E0708_RS19405	4133646..4133912	+	267	WP_012601410.1	hypothetical protein	-
E0708_RS19410	4133901..4134239	-	339	WP_000394541.1	hypothetical protein	-
E0708_RS19415	4134251..4134403	-	153	WP_000380313.1	DUF1391 family protein	-
E0708_RS19420	4134695..4134973	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E0708_RS19425	4134975..4135166	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E0708_RS19430	4135187..4135558	-	372	WP_001169687.1	hypothetical protein	-
E0708_RS19435	4135656..4135958	+	303	WP_001556930.1	transcriptional regulator	-
E0708_RS19440	4135955..4136380	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E0708_RS19445	4136403..4137365	+	963	WP_000095673.1	helix-turn-helix domain-containing protein	-
E0708_RS19450	4137372..4138118	+	747	WP_000788950.1	ATP-binding protein	-
E0708_RS19455	4138140..4138910	+	771	WP_000450998.1	DUF1627 domain-containing protein	-
E0708_RS19460	4138926..4139348	+	423	Protein_3791	DUF977 family protein	-
E0708_RS19465	4139454..4139666	+	213	WP_000072553.1	hypothetical protein	-
E0708_RS19470	4139699..4139917	+	219	WP_000209148.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4121656..4219620	97964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T119971 WP_001303511.1 NZ_CP037449:c4134973-4134695 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T119971 NZ_CP037449:c4134973-4134695 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT119971 WP_001302048.1 NZ_CP037449:c4135166-4134975 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT119971 NZ_CP037449:c4135166-4134975 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0