Detailed information of TA system TA119970

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4039978..4040812
• Replicon: chromosome
• Accession: NZ_CP037449
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: E0708_RS18905
• Protein ID: WP_000854690.1
• Coordinates: 4040435..4040812 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: E0708_RS18900
• Protein ID: WP_001546171.1
• Coordinates: 4039978..4040346 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0708_RS18865	4036066..4036521	+	456	WP_000581502.1	hypothetical protein	-
E0708_RS24545	4036640..4036851	-	212	Protein_3674	hypothetical protein	-
E0708_RS18875	4036932..4037753	+	822	WP_001234710.1	DUF945 domain-containing protein	-
E0708_RS18880	4038016..4038489	+	474	WP_000855076.1	antirestriction protein	-
E0708_RS18885	4038505..4038981	+	477	WP_001537421.1	RadC family protein	-
E0708_RS18890	4039044..4039265	+	222	WP_000692329.1	DUF987 domain-containing protein	-
E0708_RS18895	4039284..4039928	+	645	WP_000094915.1	hypothetical protein	-
E0708_RS18900	4039978..4040346	+	369	WP_001546171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E0708_RS18905	4040435..4040812	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
E0708_RS18910	4040809..4041297	+	489	WP_001546173.1	hypothetical protein	-
E0708_RS18915	4041317..4041514	+	198	WP_000772027.1	DUF957 domain-containing protein	-
E0708_RS18920	4041599..4042426	+	828	WP_001304477.1	DUF4942 domain-containing protein	-
E0708_RS18930	4042913..4043851	+	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
E0708_RS18935	4043906..4044643	+	738	WP_000283664.1	zinc-binding phosphatase	-
E0708_RS18940	4044667..4045221	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	4015210..4050754	35544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T119970 WP_000854690.1 NZ_CP037449:4040435-4040812 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T119970 NZ_CP037449:4040435-4040812 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13745.37 Da
• Isoelectric Point: 4.6220

>AT119970 WP_001546171.1 NZ_CP037449:4039978-4040346 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT119970 NZ_CP037449:4039978-4040346 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5