Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3394832..3395450 Replicon chromosome
Accession NZ_CP037449
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E0708_RS15880 Protein ID WP_001291435.1
Coordinates 3394832..3395050 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E0708_RS15885 Protein ID WP_000344800.1
Coordinates 3395076..3395450 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0708_RS15845 3390120..3390692 + 573 WP_000779831.1 YbaY family lipoprotein -
E0708_RS15850 3390723..3391034 - 312 WP_000409908.1 MGMT family protein -
E0708_RS15860 3391413..3391766 + 354 WP_000878140.1 DUF1428 domain-containing protein -
E0708_RS15865 3391808..3393358 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E0708_RS15870 3393522..3393992 - 471 WP_001304825.1 YlaC family protein -
E0708_RS15875 3394108..3394659 - 552 WP_000102539.1 maltose O-acetyltransferase -
E0708_RS15880 3394832..3395050 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E0708_RS15885 3395076..3395450 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E0708_RS15890 3395995..3399144 - 3150 WP_169710466.1 efflux RND transporter permease AcrB -
E0708_RS15895 3399167..3400360 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T119969 WP_001291435.1 NZ_CP037449:c3395050-3394832 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T119969 NZ_CP037449:c3395050-3394832 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT119969 WP_000344800.1 NZ_CP037449:c3395450-3395076 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT119969 NZ_CP037449:c3395450-3395076 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References