Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2900928..2901478 Replicon chromosome
Accession NZ_CP037449
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag E0708_RS13595 Protein ID WP_001160966.1
Coordinates 2901164..2901478 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag E0708_RS13590 Protein ID WP_000125566.1
Coordinates 2900928..2901161 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0708_RS13565 2896001..2896288 + 288 WP_000203741.1 ferredoxin-like protein FixX -
E0708_RS13570 2896347..2897678 + 1332 WP_001183207.1 MFS transporter -
E0708_RS13575 2897786..2898316 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
E0708_RS13580 2898309..2900171 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
E0708_RS13585 2900363..2900842 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
E0708_RS13590 2900928..2901161 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
E0708_RS13595 2901164..2901478 + 315 WP_001160966.1 CcdB family protein Toxin
E0708_RS13600 2901475..2902323 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
E0708_RS13605 2902330..2902707 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
E0708_RS13610 2902710..2903531 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
E0708_RS13615 2903528..2904517 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
E0708_RS13620 2904517..2905803 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T119967 WP_001160966.1 NZ_CP037449:2901164-2901478 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T119967 NZ_CP037449:2901164-2901478 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT119967 WP_000125566.1 NZ_CP037449:2900928-2901161 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT119967 NZ_CP037449:2900928-2901161 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References