Detailed information of TA system TA119959

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 1953759..1954550
• Replicon: chromosome
• Accession: NZ_CP037449
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: E0708_RS09130
• Protein ID: WP_000691791.1
• Coordinates: 1954125..1954550 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: E0708_RS09125
• Protein ID: WP_000939437.1
• Coordinates: 1953759..1954094 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0708_RS09100	1949710..1949928	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
E0708_RS09105	1950046..1950660	-	615	WP_000772910.1	inovirus Gp2 family protein	-
E0708_RS09110	1951253..1952206	+	954	WP_000290406.1	hypothetical protein	-
E0708_RS09115	1952442..1953256	+	815	Protein_1783	DUF945 domain-containing protein	-
E0708_RS09120	1953286..1953759	+	474	WP_001292742.1	DNA repair protein RadC	-
E0708_RS09125	1953759..1954094	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E0708_RS09130	1954125..1954550	+	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
E0708_RS09135	1954571..1955762	+	1192	Protein_1787	AAA family ATPase	-
E0708_RS09140	1955747..1955968	+	222	WP_125112077.1	hypothetical protein	-
E0708_RS09145	1955965..1956696	+	732	WP_001216639.1	TIGR02646 family protein	-
E0708_RS09150	1957319..1957705	+	387	WP_001270938.1	DUF2441 domain-containing protein	-
E0708_RS09155	1958387..1958884	+	498	WP_000509808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1933097..1958884	25787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T119959 WP_000691791.1 NZ_CP037449:1954125-1954550 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T119959 NZ_CP037449:1954125-1954550 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT119959 WP_000939437.1 NZ_CP037449:1953759-1954094 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT119959 NZ_CP037449:1953759-1954094 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6