Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 1170304..1171138 | Replicon | chromosome |
| Accession | NZ_CP037449 | ||
| Organism | Escherichia coli strain ATCC 25922 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A0H2VDB0 |
| Locus tag | E0708_RS05360 | Protein ID | WP_000854688.1 |
| Coordinates | 1170761..1171138 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0H2VAY1 |
| Locus tag | E0708_RS05355 | Protein ID | WP_001285596.1 |
| Coordinates | 1170304..1170684 (+) | Length | 127 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E0708_RS05320 | 1166589..1167044 | + | 456 | WP_000581502.1 | hypothetical protein | - |
| E0708_RS05325 | 1167123..1167356 | + | 234 | WP_001119727.1 | DUF905 family protein | - |
| E0708_RS05330 | 1167457..1168275 | + | 819 | WP_001234616.1 | DUF945 domain-containing protein | - |
| E0708_RS05335 | 1168330..1168815 | + | 486 | WP_000849564.1 | antirestriction protein | - |
| E0708_RS05340 | 1168831..1169307 | + | 477 | WP_001186726.1 | RadC family protein | - |
| E0708_RS05345 | 1169370..1169591 | + | 222 | WP_000692329.1 | DUF987 domain-containing protein | - |
| E0708_RS05350 | 1169610..1170254 | + | 645 | WP_000094917.1 | hypothetical protein | - |
| E0708_RS05355 | 1170304..1170684 | + | 381 | WP_001285596.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| E0708_RS05360 | 1170761..1171138 | + | 378 | WP_000854688.1 | TA system toxin CbtA family protein | Toxin |
| E0708_RS05365 | 1171135..1171623 | + | 489 | WP_000761701.1 | hypothetical protein | - |
| E0708_RS05370 | 1171640..1171837 | + | 198 | WP_000839260.1 | DUF957 domain-containing protein | - |
| E0708_RS05375 | 1172051..1173070 | + | 1020 | WP_000875213.1 | IS110 family transposase | - |
| E0708_RS05380 | 1173354..1174199 | + | 846 | WP_001280441.1 | DUF4942 domain-containing protein | - |
| E0708_RS05385 | 1174268..1174663 | + | 396 | WP_000208383.1 | type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein | - |
| E0708_RS05390 | 1174656..1175590 | + | 935 | Protein_1052 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / kpsT / kpsM / gspM | 1170304..1195897 | 25593 | |
| - | flank | IS/Tn | - | - | 1172051..1173070 | 1019 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14014.01 Da Isoelectric Point: 8.5221
>T119957 WP_000854688.1 NZ_CP037449:1170761-1171138 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR
Download Length: 378 bp
>T119957 NZ_CP037449:1170761-1171138 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA
Antitoxin
Download Length: 127 a.a. Molecular weight: 13897.70 Da Isoelectric Point: 4.7959
>AT119957 WP_001285596.1 NZ_CP037449:1170304-1170684 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE
MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE
Download Length: 381 bp
>AT119957 NZ_CP037449:1170304-1170684 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCGCCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCGCCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H2VDB0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H2VAY1 |