Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1003268..1003922 Replicon chromosome
Accession NZ_CP037449
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag E0708_RS04500 Protein ID WP_000244781.1
Coordinates 1003268..1003675 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag E0708_RS04505 Protein ID WP_000354046.1
Coordinates 1003656..1003922 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0708_RS04480 999225..1000958 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
E0708_RS04485 1000964..1001674 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
E0708_RS04490 1001699..1002595 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
E0708_RS04495 1002707..1003228 + 522 WP_001055874.1 flavodoxin FldB -
E0708_RS04500 1003268..1003675 - 408 WP_000244781.1 protein YgfX Toxin
E0708_RS04505 1003656..1003922 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
E0708_RS04510 1004165..1005145 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
E0708_RS04515 1005222..1005881 - 660 WP_000250274.1 hemolysin III family protein -
E0708_RS04520 1006045..1006356 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
E0708_RS04525 1006401..1007834 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T119956 WP_000244781.1 NZ_CP037449:c1003675-1003268 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T119956 NZ_CP037449:c1003675-1003268 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT119956 WP_000354046.1 NZ_CP037449:c1003922-1003656 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT119956 NZ_CP037449:c1003922-1003656 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References