Detailed information of TA system TA119932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1641094..1641731
• Replicon: chromosome
• Accession: NZ_CP036518
• Organism: Bacillus velezensis strain ANSB01E

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: EYB46_RS07805
• Protein ID: WP_003156187.1
• Coordinates: 1641094..1641444 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: EYB46_RS07810
• Protein ID: WP_003156188.1
• Coordinates: 1641450..1641731 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB46_RS07765	1636134..1636736	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
EYB46_RS07770	1636736..1637524	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
EYB46_RS07775	1637490..1637972	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
EYB46_RS07780	1637969..1638298	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
EYB46_RS07785	1638362..1639369	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
EYB46_RS07790	1639381..1639782	-	402	WP_003156178.1	anti-sigma regulatory factor	-
EYB46_RS07795	1639785..1640150	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
EYB46_RS07800	1640155..1640976	-	822	WP_003156182.1	STAS domain-containing protein	-
EYB46_RS07805	1641094..1641444	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
EYB46_RS07810	1641450..1641731	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
EYB46_RS07815	1641851..1643020	-	1170	WP_032873001.1	alanine racemase	-
EYB46_RS07820	1643137..1644144	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
EYB46_RS07825	1644309..1644674	-	366	WP_007609580.1	holo-ACP synthase	-
EYB46_RS07830	1644767..1645366	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T119932 WP_003156187.1 NZ_CP036518:c1641444-1641094 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T119932 NZ_CP036518:c1641444-1641094 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT119932 WP_003156188.1 NZ_CP036518:c1641731-1641450 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT119932 NZ_CP036518:c1641731-1641450 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5