Detailed information of TA system TA119912

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 777564..778225
• Replicon: chromosome
• Accession: NZ_CP036514
• Organism: Hafnia alvei strain PCM_1220

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: S3IMJ6
• Locus tag: EYZ02_RS03705
• Protein ID: WP_000698542.1
• Coordinates: 777902..778225 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: S3J179
• Locus tag: EYZ02_RS03700
• Protein ID: WP_000065326.1
• Coordinates: 777564..777881 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYZ02_RS03655	772704..773528	+	825	WP_000197385.1	DUF945 domain-containing protein	-
EYZ02_RS03660	773737..774447	+	711	WP_024198336.1	hypothetical protein	-
EYZ02_RS03665	774473..775009	+	537	WP_000219797.1	DUF4339 domain-containing protein	-
EYZ02_RS03670	775051..775488	+	438	WP_024198335.1	hypothetical protein	-
EYZ02_RS03675	775555..775965	+	411	WP_000912997.1	hypothetical protein	-
EYZ02_RS03680	776043..776279	+	237	WP_000004273.1	DUF905 domain-containing protein	-
EYZ02_RS03685	776365..776823	+	459	WP_016538084.1	antirestriction protein	-
EYZ02_RS03690	776839..777315	+	477	WP_000811694.1	RadC family protein	-
EYZ02_RS03695	777324..777545	+	222	WP_000691995.1	DUF987 domain-containing protein	-
EYZ02_RS03700	777564..777881	+	318	WP_000065326.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EYZ02_RS03705	777902..778225	+	324	WP_000698542.1	TA system toxin CbtA family protein	Toxin
EYZ02_RS03710	778520..779152	-	633	WP_025801609.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
EYZ02_RS03715	779162..780091	-	930	WP_131390599.1	phosphatidate cytidylyltransferase	-
EYZ02_RS03720	780084..780686	-	603	WP_025801607.1	CDP-alcohol phosphatidyltransferase family protein	-
EYZ02_RS03725	781150..781686	-	537	WP_046359321.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	762385..788454	26069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12350.38 Da
• Isoelectric Point: 8.9789

>T119912 WP_000698542.1 NZ_CP036514:777902-778225 [Hafnia alvei]
MKILPATISRAAKPCLPPVAVWQLLLTRLLEKHYGLTLNDTPFSDETVIKEHFDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTNIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T119912 NZ_CP036514:777902-778225 [Hafnia alvei]
ATGAAAATTCTACCGGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGCCACCCGTTGCAGTCTGGCAATTGCTTCTTAC
ACGGCTACTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCAGTGATGAAACCGTTATAAAGGAACATTTCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGAATATTGATATCATGAGGGCCCGCCGCGATCTGGGTTTATTAAATCGTAA
TTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11830.50 Da
• Isoelectric Point: 6.9367

>AT119912 WP_000065326.1 NZ_CP036514:777564-777881 [Hafnia alvei]
MSNIIWGLQRDITPRLGTRLVQEGNQLHYLADRACITGKFSDAECLKLDVAFPHFISRMESMLSTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT119912 NZ_CP036514:777564-777881 [Hafnia alvei]
ATGAGCAACATCATTTGGGGCCTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTACCTGGCTGACCGGGCCTGTATCACAGGTAAGTTCAGTGATGCCGAATGTCTTAAGCTGGATGTGGCATTTCCGC
ATTTTATCAGCCGGATGGAATCCATGCTGAGCACGGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGTGGCTATGTATACGTCGCTGTTTATTCCACTCAGCGCTAA

Structures

Toxin

• AlphaFold DB: S3IMJ6

Antitoxin

• AlphaFold DB: S3J179