119906

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 401262..401821 Replicon chromosome
Accession NZ_CP036500
Organism Vibrio cholerae strain 20000

Toxin (Protein)


Gene name relE Uniprot ID D7HHK2
Locus tag EZZ25_RS16145 Protein ID WP_000578471.1
Coordinates 401262..401540 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag EZZ25_RS16150 Protein ID WP_000381183.1
Coordinates 401537..401821 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EZZ25_RS16095 396706..397305 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
EZZ25_RS16100 397362..397556 + 195 WP_131476985.1 DUF3709 domain-containing protein -
EZZ25_RS16105 397592..397810 + 219 Protein_407 DUF3709 domain-containing protein -
EZZ25_RS16110 398098..398634 + 537 WP_000469484.1 GNAT family N-acetyltransferase -
EZZ25_RS16120 399269..399538 + 270 WP_001114075.1 DUF4160 domain-containing protein -
EZZ25_RS16125 399532..400053 + 522 WP_000921691.1 DUF2442 domain-containing protein -
EZZ25_RS16130 400352..400477 + 126 WP_001905700.1 DUF645 family protein -
EZZ25_RS16140 400728..401123 + 396 WP_001000868.1 hypothetical protein -
EZZ25_RS16145 401262..401540 - 279 WP_000578471.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
EZZ25_RS16150 401537..401821 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EZZ25_RS16155 402015..402530 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
EZZ25_RS16165 402714..403127 + 414 WP_000049420.1 VOC family protein -
EZZ25_RS16180 403682..404860 + 1179 Protein_417 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 391646..423467 31821
- inside Integron - - 322986..403614 80628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10838.56 Da        Isoelectric Point: 4.6794

>T119906 WP_000578471.1 NZ_CP036500:c401540-401262 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T119906 NZ_CP036500:c401540-401262 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TGAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATGGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT119906 WP_000381183.1 NZ_CP036500:c401821-401537 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT119906 NZ_CP036500:c401821-401537 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VKS2

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References